Goat anti-Chicken IgM Secondary Antibody

• Reactivity: Ch
• Applications: WB, ELISA, ICC/IF, IHC, IHC-P
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 1.0 mg/ml

Goat anti-Chicken IgM Secondary Antibody Summary

• Immunogen: Chicken IgM
• Specificity: By immunoelectrophoresis and ELISA this reacts specifically with chicken IgM. This may cross react with IgM from other species.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: IGHM
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:1000 - 1:30000
  • ELISA 1:1000-1:30000
  • Immunocytochemistry/Immunofluorescence 1:200 - 1:2000
  • Immunohistochemistry 1:200 - 1:2000
  • Immunohistochemistry-Paraffin 1:200 - 1:2000

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Phosphate Buffered Saline (PBS)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Goat anti-Chicken IgM Secondary Antibody

• AGM1
• DKFZp686I15196
• DKFZp686I15212
• FLJ00385
• IgM
• immunoglobulin heavy constant mu
• MGC104996
• MGC52291
• MU
• VH

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IgM Antibody (NB7276)(36)

Publications using NB7276

• Sale JE. Measurement of diversification in the immunoglobulin light chain gene of DT40 cells. Methods Mol Biol. 2012 [PMID: 22941620]
  
  Details:
  Citation using the FITC form of this antibody.
• Lipchik AM, Killins RL, Geahlen RL et al. A Peptide-Based Biosensor Assay To Detect Intracellular Syk Kinase Activation and Inhibition. Biochemistry 2012 Sep [PMID: 22920457]
• Lin W, Kurosawa K, Murayama A et al. B-cell display-based one-step method to generate chimeric human IgG monoclonal antibodies. Nucleic Acids Res. [PMID: 21062829]
  
  Details:
  Citation using the HRP form of this antibody.
• Numaga T, Nishida M, Kiyonaka S et al. Ca2+ influx and protein scaffolding via TRPC3 sustain PKCbeta and ERK activation in B cells. J Cell Sci. [PMID: 20179100]
  
  Details:
  Citation using the FITC form of this antibody.
• Lai HT, NieuwlMG, Kemp B et al. Effects of dust and airborne dust components on antibody responses, body weight gain, and heart morphology of broilers. Poult Sci. [PMID: 19687268]
  
  Details:
  Citation using the HRP form of this antibody.
• Imamura Y, Oda A, Katahira T et al. BLNK binds active H-Ras to promote B cell receptor-mediated capping and ERK activation. J Biol Chem. [PMID: 19218240]
  
  Details:
  Citation using the FITC form of this antibody.
• Volna P, Jarjour J, Baxter S et al. Flow cytometric analysis of DNA binding and cleavage by cell surface-displayed homing endonucleases. Nucleic Acids Res. 2007 [PMID: 17426121]
  
  Details:
  Citation using the FITC form of this antibody.
• Shinohara H, Yasuda T, Aiba Y et al. PKC beta regulates BCR-mediated IKK activation by facilitating the interaction between TAK1 and CARMA1. J Exp Med 2005 Nov [PMID: 16301747]
• Verminnen K, Loock MV, Cox E et al. Protection of turkeys against Chlamydophila psittaci challenge by DNA and rMOMP vaccination and evaluation of the immunomodulating effect of 1 alpha,25-dihydroxyvitamin D(3). Vaccine. [PMID: 16002193]
  
  Details:
  Citation using the HRP form of this antibody.
• Seo H, Masuoka M, Murofushi H et al. Rapid generation of specific antibodies by enhanced homologous recombination. Nat Biotechnol. [PMID: 15924134]
  
  Details:
  Citation using the HRP form of this antibody.
• Serrano CJ, Graham L, DeBell K et al. A new tyrosine phosphorylation site in PLC gamma 1: the role of tyrosine 775 in immune receptor signaling. J Immunol 2005 May [PMID: 15879121]
• Hao S, August A. Actin depolymerization transduces the strength of B-cell receptor stimulation. Mol Biol Cell 2005 May [PMID: 15728723]
• Simpson LJ, Sale JE. UBE2V2 (MMS2) is not required for effective immunoglobulin gene conversion or DNA damage tolerance in DT40. DNA Repair (Amst). [PMID: 15725630]
  
  Details:
  Citation using the FITC form of this antibody.
• Stoddart A, Jackson AP, Brodsky FM et al. Plasticity of B cell receptor internalization upon conditional depletion of clathrin. Mol Biol Cell 2005 May [PMID: 15716350]
• Niiro H, Allam A, Stoddart A et al. The B lymphocyte adaptor molecule of 32 kilodaltons (Bam32) regulates B cell antigen receptor internalization. J Immunol 2004 Nov [PMID: 15494510]
• Hao JJ, Carey GB, Zhan X et al. Syk-mediated tyrosine phosphorylation is required for the association of hematopoietic lineage cell-specific protein 1 with lipid rafts and B cell antigen receptor signalosome complex. J Biol Chem 2004 Aug [PMID: 15166239]
• Chen J, McLean PA, Neel BG et al. CD22 attenuates calcium signaling by potentiating plasma membrane calcium-ATPase activity. Nat Immunol. [PMID: 15133509]
  
  Details:
  Citation using the FITC form of this antibody.
• Oh-hora M, Johmura S, Hashimoto A et al. Requirement for Ras guanine nucleotide releasing protein 3 in coupling phospholipase C-gamma2 to Ras in B cell receptor signaling. J Exp Med. [PMID: 14676298]
  
  Details:
  Citation using the FITC form of this antibody.
• Spaargaren M, Beuling EA, Rurup ML et al. The B cell antigen receptor controls integrin activity through Btk and PLCgamma2. J Exp Med 2003 Nov [PMID: 14610042]
• Berthelot-Herault F, Mompart F, Zygmunt MS et al. Antibody responses in the serum and gut of chicken lines differing in cecal carriage of Salmonella enteritidis. Vet Immunol Immunopathol. [PMID: 14522133]
  
  Details:
  Citation using the HRP form of this antibody.
• Lammers A, Klomp ME, NieuwlMG et al. Adoptive transfer of natural antibodies to non-immunized chickens affects subsequent antigen-specific humoral and cellular immune responses. Dev Comp Immunol. [PMID: 12962982]
  
  Details:
  Citation using the HRP form of this antibody.
• Parmentier HK, Lammers A, Hoekman JJ et al. Different levels of natural antibodies in chickens divergently selected for specific antibody responses. Dev Comp Immunol. [PMID: 12962981]
  
  Details:
  Citation using the HRP form of this antibody.
• Rellahan BL, Graham LJ, Tysgankov AY et al. A dynamic constitutive and inducible binding of c-Cbl by PLCgamma1 SH3 and SH2 domains (negatively) regulates antigen receptor-induced PLCgamma1 activation in lymphocytes. Exp Cell Res 2003 Sep [PMID: 12941616]
• Johmura S, Oh-hora M, Inabe K et al. Regulation of Vav localization in membrane rafts by adaptor molecules Grb2 and BLNK. Immunity. [PMID: 12818159]
  
  Details:
  Citation using the FITC form of this antibody.
• Ono M, Okuda Y, Yazawa S et al. Adenoviral gizzard erosion in commercial broiler chickens. Vet Pathol 2003 May [PMID: 12724571]
• Simpson LJ, Sale JE. Rev1 is essential for DNA damage tolerance and non-templated immunoglobulin gene mutation in a vertebrate cell line. EMBO J. [PMID: 12660171]
  
  Details:
  Citation using the FITC form of this antibody.
• Cumbers SJ, Williams GT, Davies SL et al. Generation and iterative affinity maturation of antibodies in vitro using hypermutating B-cell lines. Nat Biotechnol. [PMID: 12379869]
  
  Details:
  Citation using the FITC form of this antibody.
• Hong JJ, Yankee TM, Harrison ML et al. Regulation of signaling in B cells through the phosphorylation of Syk on linker region tyrosines. A mechanism for negative signaling by the Lyn tyrosine kinase. J Biol Chem. [PMID: 12077122]
  
  Details:
  Citation using the FITC form of this antibody.
• Harris RS, Sale JE, Petersen-Mahrt SK et al. AID is essential for immunoglobulin V gene conversion in a cultured B cell line. Curr Biol. [PMID: 11882297]
  
  Details:
  Citation using the FITC form of this antibody.
• Niiro H, Maeda A, Kurosaki T et al. The B lymphocyte adaptor molecule of 32 kD (Bam32) regulates B cell antigen receptor signaling and cell survival. J Exp Med. [PMID: 11781373]
  
  Details:
  Citation using the FITC form of this antibody.
• Sale JE, Calandrini DM, Takata M et al. Ablation of XRCC2/3 transforms immunoglobulin V gene conversion into somatic hypermutation. Nature. [PMID: 11528482]
  
  Details:
  Citation using the FITC form of this antibody.
• Goitsuka R, Tatsuno A, Ishiai M et al. MIST functions through distinct domains in immunoreceptor signaling in the presence and absence of LAT. J Biol Chem. [PMID: 11463797]
  
  Details:
  Citation using the FITC form of this antibody.
• Fukutome K, Watarai S, Mukamoto M et al. Intestinal mucosal immune response in chickens following intraocular immunization with liposome-associated Salmonella enterica serovar enteritidis antigen. Dev Comp Immunol. [PMID: 11356227]
  
  Details:
  Citation using the HRP form of this antibody.
• Ishiai M, Kurosaki M, Inabe K et al. Involvement of LAT, Gads, and Grb2 in compartmentation of SLP-76 to the plasma membrane. J Exp Med. [PMID: 10993915]
  
  Details:
  Citation using the FITC form of this antibody.
• Klipper E, Sklan D, Friedman A et al. Immune responses of chickens to dietary protein antigens. I. Induction of systemic and intestinal immune responses following oral administration of soluble proteins in the absence of adjuvant. Vet Immunol Immunopathol. [PMID: 10802289]
  
  Details:
  Citation using the HRP form of this antibody.
• Yasuda T, Maeda A, Kurosaki M et al. Cbl suppresses B cell receptor-mediated phospholipase C (PLC)-gamma2 activation by regulating B cell linker protein-PLC-gamma2 binding. J Exp Med. [PMID: 10684856]
  
  Details:
  Citation using the FITC form of this antibody.

Bioinformatics

• Gene Symbol: IGHM