Goat anti-Mouse Kappa Light Chain Secondary Antibody [HRP] (Azide Free)

Product Discontinued

Goat anti-Mouse Kappa Light Chain Secondary Antibody [HRP] (Azide Free) Summary

• Immunogen: A pool of purified Bence Jones kappa proteins isolated from mouse urine. Freund's complete adjuvant is used in the first step of the immunization procedure.
• Specificity: Horseradish peroxidase-conjugated IgG fraction of polyclonal Goat antiSerum to Mouse Ig kappa chain, free and bound
• Clonality: Polyclonal
• Host: Goat
• Gene: IGKC
• Purity: Ion exchange chromatography

Applications/Dilutions

• Dilutions:
  • Dot Blot 1:100-1:2000
  • ELISA 1:100-1:2000
  • Immunoblotting 1:100 - 1:2000
  • Immunocytochemistry/ Immunofluorescence 1:10 - 1:500
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:10 - 1:500
• Application Notes: .

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from PBS (pH 7.2)
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Ion exchange chromatography
• Reconstitution Instructions: It is reconstituted by adding 1 ml sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20C. Prior to use, an aliquot is thawed slowly in the dark at ambient temperature, spun down a

Alternate Names for Goat anti-Mouse Kappa Light Chain Secondary Antibody [HRP] (Azide Free)

• HCAK1
• IGKCD
• immunoglobulin kappa constant
• Km
• MGC111575
• MGC62011
• MGC72072
• MGC88770
• MGC88771
• MGC88809

Background

Kappa light chain antibody can be used for the identification of leukemias, plasmacytomas and certain non-Hodgkin's lymphomas. Kappa light chain contains one immunoglobulin-like domain. The EU sequence has the INV allotypic marker, Ala-45 and Val-83. The ROY sequence has the INV allotypic marker, Ala-45 and Leu-83.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: IGKC