Human DPPIV/CD26 DuoSet ELISA, 15 Plate

• Reactivity: Hu
• Applications: ELISA
• Conjugate: Biotin

Human DPPIV/CD26 DuoSet ELISA, 15 Plate Summary

• Source: N/A
• Assay Type: Solid Phase Sandwich ELISA
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details
• Gene: DPP4

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: No significant interference observed with available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Human DPPIV/CD26 DuoSet ELISA, 15 Plate

• ADABP
• ADCP-2
• ADCP2DPP IV
• Adenosine deaminase complexing protein 2TP103
• CD26 antigen
• CD26
• CD26T-cell activation antigen CD26
• dipeptidyl peptidase 4
• Dipeptidyl peptidase IV
• dipeptidylpeptidase 4
• dipeptidyl-peptidase 4
• dipeptidylpeptidase IV (CD26, adenosine deaminase complexing protein 2)
• DPP4
• DPPIV
• EC 3.4.14.5

Background

DPPIV/CD26 is a serine exopeptidase that is expressed as a noncovalent homodimer on the surface of epithelial cells, endothelial cells, and activated lymphocytes. It regulates multiple aspects of immune and endocrine function by cleaving Xaa-Pro or Xaa-Ala dipeptides from the N-terminus of a wide variety of chemokines (CCL4 and 5, CXCL6, 9, 10, 11, and 12), growth factors (GM-CSF, IL-3), and peptide hormones (Glucagon, Glucagon-like Peptides 1 and 2, GIP, GHRH, Procalcitonin, Neuropeptide Y, and Substance P). DPPIV interacts in cis with Adenosine Deaminase on T cells, in trans with Caveolin-1 on antigen presenting cells, and it serves as a cell entry coreceptor for HIV and coronavirus. It provides costimulatory proliferation and activation signals to both CD4+ and CD8+ T cells. A soluble form of DPPIV can be proteolytically shed from adipocytes, leading to insulin resistance in adipocytes and skeletal muscle.

Publications for DPPIV/CD26 (DY1180)(14)

Publications using DY1180

• Höpfinger, A;Karrasch, T;Schäffler, A;Schmid, A; Circulating Concentrations of Cathelicidin Anti-Microbial Peptide (CAMP) Are Increased during Oral Glucose Tolerance Test International journal of molecular sciences 2023-08-17 [PMID: 37629082] (Human)
• Höpfinger, A;Karrasch, T;Schäffler, A;Schmid, A; Circulating Levels of Cathelicidin Antimicrobial Peptide (CAMP) Are Affected by Oral Lipid Ingestion Nutrients 2023-07-03 [PMID: 37447348] (Human)
• Kun Li, Christine Wohlford-Lenane, Jennifer A. Bartlett, Paul B. McCray Inter-individual Variation in Receptor Expression Influences MERS-CoV Infection and Immune Responses in Airway Epithelia Frontiers in Public Health 2022-01-04 [PMID: 35059374] (Human)
• OJ Cordero, C Rafael-Vid, R Varela-Cal, C Calviño-Sa, B Malvar-Fer, S García, JE Viñuela, JM Pego-Reigo Distinctive CD26 Expression on CD4 T-Cell Subsets Biomolecules, 2021-10-02;11(10):. 2021-10-02 [PMID: 34680079] (Human)
• April Rees, Steve Turner, Catherine A. Thornton Potential protective effects of breast milk and amniotic fluid against novel coronavirus SARS‐CoV‐2 through decoy receptors Pediatric Allergy and Immunology 2022-01-01 [PMID: 34585801] (Human)
• E Krepela, Z Vanickova, P Hrabal, M Zubal, B Chmielova, E Balaziova, P Vymola, I Matrasova, P Busek, A Sedo Regulation of Fibroblast Activation Protein by Transforming Growth Factor Beta-1 in Glioblastoma Microenvironment International Journal of Molecular Sciences, 2021-01-21;22(3):. 2021-01-21 [PMID: 33494271] (Human)
• AA Raha, S Chakrabort, J Henderson, E Mukaetova-, S Zaman, J Trowsdale, R Raha-Chowd Investigation of CD26, a potential SARS-CoV-2 receptor, as a biomarker of age and pathology Biosci Rep, 2020-12-23;40(12):. 2020-12-23 [PMID: 33205807] (Human)
• LL Baggio, EM Varin, JA Koehler, X Cao, Y Lokhnygina, SR Stevens, RR Holman, DJ Drucker Plasma levels of DPP4 activity and sDPP4 are dissociated from inflammation in mice and humans Nat Commun, 2020-07-28;11(1):3766. 2020-07-28 [PMID: 32724076] (Human)
• NM Varda, M Medved, L Ojsteršek The associations between some biological markers, obesity, and cardiovascular risk in Slovenian children and adolescents BMC Pediatr, 2020-02-21;20(1):81. 2020-02-21 [PMID: 32085704] (Human)
• L De Chiara, M Páez de la, J Rodríguez-, MC Alvarez-Pa, MC Pardiñas-A, R Varela-Cal, OJ Cordero CD26-Related Serum Biomarkers: sCD26 Protein, DPP4 Activity, and Anti-CD26 Isotype Levels in a Colorectal Cancer-Screening Context Dis. Markers, 2020-01-21;2020(0):4347936. 2020-01-21 [PMID: 32051696] (Human)
• MJ Ploquin, A Casrouge, Y Madec, N Noël, B Jacquelin, N Huot, D Duffy, SP Jochems, L Micci, C Lécuroux, F Boufassa, T Booiman, T Garcia-Tel, M Ghislain, RL Grand, O Lambotte, N Kootstra, L Meyer, C Goujard, M Paiardini, ML Albert, M Müller-Tru Systemic DPP4 activity is reduced during primary HIV-1 infection and is associated with intestinal RORC+ CD4+ cell levels: a surrogate marker candidate of HIV-induced intestinal damage J Int AIDS Soc, 2018-07-01;21(7):e25144. 2018-07-01 [PMID: 29987877] (Human)
• Matrasova I, Busek P, Balaziova E, Sedo A Heterogeneity of molecular forms of dipeptidyl peptidase-IV and fibroblast activation protein in human glioblastomas. Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub, 2017-04-26;161(3):252-260. 2017-04-26 [PMID: 28452380] (Human)
• Sromova L, Busek P, Sedova L, Sedo A Intraindividual changes of dipeptidyl peptidase-IV in peripheral blood of patients with rheumatoid arthritis are associated with the disease activity. BMC Musculoskelet Disord, 2015-09-09;16(0):244. 2015-09-09 [PMID: 26353808] (Human)
• Rohrborn D, Eckel J, Sell H Shedding of dipeptidyl peptidase 4 is mediated by metalloproteases and up-regulated by hypoxia in human adipocytes and smooth muscle cells. FEBS Lett, 2014-09-12;588(21):3870-7. 2014-09-12 [PMID: 25217834] (Human)

FAQs for DPPIV/CD26 (DY1180). (Showing 1 - 1 of 1 FAQs).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: DPP4