Human BACE-1 DuoSet ELISA, 15 Plate

• Reactivity: Hu
• Applications: ELISA
• Conjugate: Biotin

Human BACE-1 DuoSet ELISA, 15 Plate Summary

• Source: N/A
• Assay Type: Solid Phase Sandwich ELISA
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details
• Gene: BACE1

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: No significant interference observed with available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Human BACE-1 DuoSet ELISA, 15 Plate

• APP beta-secretase
• ASP2
• Aspartyl protease 2
• BACE1
• BACE-1
• BACEAsp 2
• beta-secretase 1 precursor variant 1
• beta-secretase 1
• beta-site amyloid beta A4 precursor protein-cleaving enzyme
• Beta-site amyloid precursor protein cleaving enzyme 1
• Beta-site APP cleaving enzyme 1
• beta-site APP-cleaving enzyme 1
• beta-site APP-cleaving enzyme
• EC 3.4.23
• EC 3.4.23.46
• FLJ90568
• HSPC104
• KIAA1149
• memapsin-2
• Membrane-associated aspartic protease 2
• transmembrane aspartic proteinase Asp2

Background

Both BACE-1 and BACE-2 are membrane-bound aspartic proteases of the pepsin family. BACE-1 is a potential target for treatment of Alzheimer's disease because it is the principle b-secretase responsible for cleavage of amyloid precursor protein (APP). BACE-2 differs from BACE-1 in several aspects including pro-enzyme activation, substrate binding sites, transcriptional regulation, and function in A beta peptide production.

Publications for BACE-1 (DY931)(2)

Publications using DY931

• B Gaborit, R Govers, A Altié, JM Brunel, P Morange, F Peiretti The aminosterol Claramine inhibits &beta-secretase 1-mediated insulin receptor cleavage The Journal of Biological Chemistry, 2021-05-23;0(0):100818. 2021-05-23 [PMID: 34029592] (Mouse)
• JJ Fernandez-, R Sanchez-Va, C Muñoz-Cast, V De Castro, E Sanchez-Me, V Navarro, S Jimenez, C Nuñez-Diaz, A Gomez-Arbo, I Moreno-Gon, M Vizuete, JC Davila, J Vitorica, A Gutierrez Enhancing microtubule stabilization rescues cognitive deficits and ameliorates pathological phenotype in an amyloidogenic Alzheimer's disease model Sci Rep, 2020-09-08;10(1):14776. 2020-09-08 [PMID: 32901091] (Mouse)

FAQs for BACE-1 (DY931). (Showing 1 - 1 of 1 FAQs).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: BACE1