HSPB8/HSP22 Antibody (3C5) - Azide and BSA Free

Western Blot: HSPB8/HSP22 Antibody (3C5) [H00026353-M12] - Analysis of HSPB8 expression in HeLa(Cat # L013V1).

Sandwich ELISA: HSPB8/HSP22 Antibody (3C5) [H00026353-M12] - Detection limit for recombinant GST tagged HSPB8 is 0.03 ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF
• Clone: 3C5
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

HSPB8/HSP22 Antibody (3C5) - Azide and BSA Free Summary

• Immunogen: HSPB8 (AAH02673.1, 1 a.a. ~ 196 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MADGQMPFSCHYPSRLRRDPFRDSPLSSRLLDDGFGMDPFPDDLTASWPDWALPRLSSAWPGTLRSGMVPRGPTATARFGVPAEGRTPPPFPGEPWKVCVNVHSFKPEELMVKTKDGYVEVSGKHEEKQQEGGIVSKNFTKKIQLPAEVDPVTVFASLSPEGLLIIEAPQVPPYSTFGESSFNNELPQDSQEVTCT
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: HSPB8
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Sandwich ELISA
  • Western Blot
• Application Notes: This antibody is useful for ELISA, Western Blot

Reactivity Notes

This product is reactive against Human.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for HSPB8/HSP22 Antibody (3C5) - Azide and BSA Free

• Alpha-crystallin C chain
• CMT2L
• CRYAC
• DHMN2
• E2IG1
• E2IG1E2-induced gene 1 protein
• H11
• H11DHMN2
• heat shock 22kDa protein 8
• heat shock 27kDa protein 8
• heat shock protein beta-8
• HMN2
• HMN2A
• HSP22
• HSP22CMT2L
• HSPB8
• Protein kinase H11
• Small stress protein-like protein HSP22

Background

The protein encoded by this gene belongs to the superfamily of small heat-shock proteins containing a conservative alpha-crystallin domain at the C-terminal part of the molecule. The expression of this gene in induced by estrogen in estrogen receptor-positive breast cancer cells, and this protein also functions as a chaperone in association with Bag3, a stimulator of macroautophagy. Thus, this gene appears to be involved in regulation of cell proliferation, apoptosis, and carcinogenesis, and mutations in this gene have been associated with different neuromuscular diseases, including Charcot-Marie-Tooth disease.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for HSPB8/HSP22 Antibody (H00026353-M12)(1)

Publication using H00026353-M12

• Nivon M, Fort L, Muller P et al. NFkB is a central regulator of protein quality control in response to protein aggregation stresses via autophagy modulation. Mol Biol Cell 2016-04-13 [PMID: 27075172]

Bioinformatics

• Gene Symbol: HSPB8
• Uniprot:
  • AAH02673(Human)