Hsp47 Antibody (950811) [Alexa Fluor® 405]

• Applications: WB, IHC, ICC/IF
• Clone: 950811
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 405

Hsp47 Antibody (950811) [Alexa Fluor® 405] Summary

• Immunogen: Chinese hamster ovary cell line CHO-derived recombinant human HSP47
  Ala19-Asp412
  Accession # P50454
• Specificity: Detects human HSP47 in direct ELISAs and Western blots. In Western blots, no cross-reactivity with mouse HSP47 is observed.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Background

Heat Shock Protein 47 (HSP47), also known as Serpin-H1/CBP1/CBP2, is localized to endoplasmic reticulum (ER), where it is a collagen-specific molecular chaperone.  In the ER, HSP47 interacts with and stabilizes correctly-folded procollagen. Nucleotide polymorphisms may be associated with preterm birth and Osteogenesis Imperfecta type X.  Serpin-H1 is up-regulated in several cancers including squamous cell carcinoma, breast and prostate carcinomas.  Expression in tumors drives malignant growth and invasion by enhancing deposition of extracellular matrix proteins.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.