HOXD4 Antibody (OTI1E9) - Azide and BSA Free

Western Blot: HOXD4 Antibody (OTI1E9) - Azide and BSA Free [NBP2-70919] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HOXD4 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HOXD4.

Immunohistochemistry: HOXD4 Antibody (OTI1E9) - Azide and BSA Free [NBP2-70919] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-HOXD4 mouse monoclonal antibody.

• Reactivity: Hu, Mu, Rt
• Applications: WB, IHC
• Clone: OTI1E9
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

HOXD4 Antibody (OTI1E9) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse HOXD4 Antibody (OTI1E9) - Azide and BSA Free (NBP2-46001) is a monoclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Full length human recombinant protein of human HOXD4 (NP_055436) produced in E.coli.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: HOXD4
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin
  • Western Blot 1:2000

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from PBS (pH 7.3) with 8% Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Immunogen affinity purified
• Reconstitution Instructions: we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Alternate Names for HOXD4 Antibody (OTI1E9) - Azide and BSA Free

• HHO.C13
• homeo box D4
• homeobox D4
• Homeobox protein HHO.C13
• Homeobox protein Hox-4B
• Homeobox protein Hox-5.1
• homeobox protein Hox-D4
• HOX4
• Hox-4.2
• Hox-4.2, mouse, homolog of homeo box X
• HOX4BHOX-5.1

Background

HOXD4 belongs to the homeobox family of genes. The homeobox genes encode a highly conserved family of transcription factors that play an important role in morphogenesis in all multicellular organisms. Mammals possess four similar homeobox gene clusters, HOXA, HOXB, HOXC and HOXD, located on different chromosomes, consisting of 9 to 11 genes arranged in tandem. This gene is one of several homeobox HOXD genes located at 2q31-2q37 chromosome regions. Deletions that removed the entire HOXD gene cluster or 5' end of this cluster have been associated with severe limb and genital abnormalities. The protein encoded by this gene may play a role in determining positional values in developing limb buds. Alternatively spliced variants have been described but their full length nature has not been determined. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: HOXD4