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HOXC11 Antibody

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Western Blot: HOXC11 Antibody [NBP2-87592] - WB Suggested Anti-HOXC11 Antibody Titration: 1.25ug/ml. Positive Control: Transfected 293T
Western Blot: HOXC11 Antibody [NBP2-87592] - WB Suggested Anti-HOXC11 Antibody Titration: 1.25ug/ml. Positive Control: Transfected 293T
Immunohistochemistry: HOXC11 Antibody [NBP2-87592] - Sample Type: Mouse lumbar spinal cord sections. Primary Antibody Dilution: 1:1000. Secondary Antibody: Anti-rabbit-Cy3. Secondary Antibody Dilution: 1:800. ...read more
Western Blot: HOXC11 Antibody [NBP2-87592] - Host: Rabbit. Target Name: HOXC11. Sample Tissue: Human RPMI 8226 Whole Cell. Antibody Dilution: 1ug/ml

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

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HOXC11 Antibody Summary

Immunogen
The immunogen is a synthetic peptide directed towards the middle region of human HOXC11. Peptide sequence: DRFFDNAYCGGGDPPAEPPCSGKGEAKGEPEAPPASGLASRAEAGAEAEA The peptide sequence for this immunogen was taken from within the described region.
Clonality
Polyclonal
Host
Rabbit
Gene
HOXC11
Purity
Protein A purified
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Applications/Dilutions

Dilutions
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot 1.0 ug/ml

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS, 2% Sucrose
Preservative
0.09% Sodium Azide
Purity
Protein A purified

Alternate Names for HOXC11 Antibody

  • homeo box C11
  • homeobox C11
  • Homeobox protein Hox-3H
  • homeobox protein Hox-C11
  • HOX3H
  • MGC4906

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Species: Hu, Mu, Rt
Applications: WB
HOXA11 Antibody
NBP1-80228
Immunohistochemistry-Paraffin: HOXA11 Antibody [NBP1-80228] - Human Uterus Tissue, 4-8ug/ml.
Western Blot: HOXA11 Antibody [NBP1-80228] - Titration: 0.2-1 ug/ml, Positive Control: Human Uterus.
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Applications: IHC, IHC-P, WB
HOXD11 Antibody
(6C8)
H00003237-M10
Western Blot: HOXD11 Antibody (6C8) [H00003237-M10] - Analysis of HOXD11 expression in K-562 (Cat # L009V1).
Immunocytochemistry/Immunofluorescence: HOXD11 Antibody (6C8) [H00003237-M10] - Analysis of monoclonal antibody to HOXD11 on HeLa cell. Antibody concentration 10 ug/ml
Immunohistochemistry-Paraffin: HOXD11 Antibody (6C8) [H00003237-M10] - Analysis of monoclonal antibody to HOXD11 on formalin-fixed paraffin-embedded human colon. Antibody concentration 0.7 ug/ml
Species: Hu
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S100B Antibody
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Flow Cytometry: S100B Antibody (15F9NB) [NBP2-45267] - An intracellular stain was performed on SK-MEL-28 cells with S100B [15F9NB] Antibody NBP2-45267PE (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Phycoerythrin.
Flow Cytometry: S100B Antibody (15F9NB) [NBP2-45267] - Staining of A431 cells (1 x 10^6 cells/ml) with S100B antibody, clone 15F4.1D2 (Orange) stained at a dilution of 1:500. Detected with a GtxRb DyLight 488 secondary. Shown with the secondary control (Blue).
Immunocytochemistry/Immunofluorescence: S100B Antibody (15F9NB) [NBP2-45267] - Analysis in A431 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were incubated with S100B (15F4NB) at a 1:100 dilution overnight at 4 degrees Celsius and detected with DyLight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were detected with DAPI (Blue) at 2.0 ug/ml in 1X PBS. Cells were imaged using a 40X objective.
Species: Hu
Applications: Flow, ICC/IF, IHC, IHC-P, WB
Protamine 1
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Immunohistochemistry-Paraffin: Protamine 1 Antibody [NBP2-30949] - Staining of human testis shows high expression.
Immunohistochemistry-Paraffin: Protamine 1 Antibody [NBP2-30949] - Staining of human endometrium shows no positivity in glandular cells as expected.
Immunohistochemistry-Paraffin: Protamine 1 Antibody [NBP2-30949] - Staining in human testis and endometrium tissues.. Corresponding PRM1 RNA-seq data are presented for the same tissues.
Species: Hu
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SRC1 Antibody
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(1 Review)
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Applications: ChIP, IP, WB
Oct-3/4 Antibody
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BG01V human embryonic stem cells were fixed using formaldehyde, resuspended in lysis buffer, and sonicated to shear chromatin. Oct‑3/4/DNA complexes were immunoprecipitated using 5 μg Goat Anti-Human/Mouse Oct‑3/4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1759) or control antibody (Catalog # AB-108-C) for 15 minutes in an ultrasonic bath, followed by Biotinylated Anti-Goat IgG Secondary Antibody (Catalog # BAF109). Immunocomplexes were captured using 50 μL of MagCellect Streptavidin Ferrofluid (Catalog # MAG999) and DNA was purified using chelating resin solution. The nanog promoter was detected by standard PCR.
Alkaline phosphatase (ALPL) and Oct‑3/4 were detected in human BG01V embryonic stem cells using 10 µg/mL Human/Mouse/Rat ALPL Monoclonal Antibody (Catalog # MAB1448) and 10 µg/mL Goat Anti-Human/Mouse Oct‑3/4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1759). Cells were incubated with primary antibodies for 3 hours at room temperature. Cells were stained for ALPL using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (pseudo-stained green; Catalog # NL007), and stained for Oct-3/4 using the NorthernLights 637-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL002). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Simple Western lane view shows lysates of BG01V human embryonic stem cells and NTera‑2 human testicular embryonic carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for Oct‑3/4 at approximately 50 kDa (as indicated) using 20 µg/mL of Goat Anti-Human/Mouse Oct‑3/4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1759) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
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Western Blot: SIX2 Antibody (3D7) [H00010736-M01] - Analysis of SIX2 expression in transfected 293T cell line by SIX2 monoclonal antibody (M01), clone 3D7.Lane 1: SIX2 transfected lysate(32.3 KDa).Lane 2: Non-transfected lysate.
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HOXC10 Antibody
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Species: Hu, Mu, Rt
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HOXC12 Antibody
(1C6)
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Western Blot: HOXC12 Antibody (1C6) [H00003228-M01] - HOXC12 monoclonal antibody (M01), clone 1C6. Analysis of HOXC12 expression in NIH/3T3.
Sandwich ELISA: HOXC12 Antibody (1C6) [H00003228-M01] - Detection limit for recombinant GST tagged HOXC12 is approximately 0.1ng/ml as a capture antibody.
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p15INK4b/CDKN2B
Antibody (OTI3B6)
NBP2-45603
Flow Cytometry: p15INK4b/CDKN2B Antibody (OTI3B6) [NBP2-45603] - Analysis of HEK293T cells transfected with either overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by CDKN2B antibody.
Immunohistochemistry: p15INK4b/CDKN2B Antibody (OTI3B6) [NBP2-45603] - Analysis of Human pancreas tissue.
Immunohistochemistry: p15INK4b/CDKN2B Antibody (OTI3B6) [NBP2-45603] - Analysis of Carcinoma of Human thyroid tissue.
Species: Hu, Mu, Rt
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NUP98 Antibody
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(4 Publications)
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SUB1 Antibody
NBP1-82454
Immunohistochemistry-Paraffin: SUB1 Antibody [NBP1-82454] - Staining of human skeletal muscle shows moderate nuclear positivity in myocytes.
Immunohistochemistry-Paraffin: SUB1 Antibody [NBP1-82454] - Staining of human rectum shows strong nuclear positivity in glandular cells.
Immunohistochemistry-Paraffin: SUB1 Antibody [NBP1-82454] - Staining of human tonsil shows strong nuclear positivity in non - germinal center cells.
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Western Blot: HOXD4 Antibody (OTI1E9) [NBP2-46001] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HOXD4 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HOXD4.
Immunohistochemistry-Paraffin: HOXD4 Antibody (OTI1E9) [NBP2-46001] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-HOXD4 mouse monoclonal antibody.
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HNF1 Antibody
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Western Blot: HNF1 Antibody [NBP1-33596] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HNF1 alpha antibody [N1N3] diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Western Blot: HNF1 Antibody [NBP1-33596] - Analysis. Mouse tissue extracts (50 Ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with HNF1 alpha antibody [N1N3] diluted at 1:2000.
Western Blot: HNF1 Antibody [NBP1-33596] - Rat tissue extracts (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with HNF1 alpha antibody [N1N3] diluted at 1:1000.
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Species: Hu, Mu, Rt
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Bioinformatics

Gene Symbol HOXC11