HOXC10 Antibody (OTI5C10) - Azide and BSA Free

Western Blot: HOXC10 Antibody (OTI5C10) - Azide and BSA Free [NBP2-70909] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HOXC10 .

Immunohistochemistry: HOXC10 Antibody (OTI5C10) - Azide and BSA Free [NBP2-70909] - Analysis of Human liver tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)(1:150)

Immunohistochemistry: HOXC10 Antibody (OTI5C10) - Azide and BSA Free [NBP2-70909] - Analysis of Adenocarcinoma of Human breast tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)(1:150)

Immunohistochemistry: HOXC10 Antibody (OTI5C10) - Azide and BSA Free [NBP2-70909] - Analysis of Carcinoma of Human kidney tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)(1:150)

Immunohistochemistry: HOXC10 Antibody (OTI5C10) - Azide and BSA Free [NBP2-70909] - Analysis of Carcinoma of Human prostate tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)(1:150)

Immunohistochemistry: HOXC10 Antibody (OTI5C10) - Azide and BSA Free [NBP2-70909] - Analysis of Human bladder tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)(1:150)

Immunohistochemistry: HOXC10 Antibody (OTI5C10) - Azide and BSA Free [NBP2-70909] - Analysis of Human Kidney tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)(1:150)

Immunohistochemistry: HOXC10 Antibody (OTI5C10) - Azide and BSA Free [NBP2-70909] - Analysis of Human prostate tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)(1:150)

• Reactivity: Hu, Mu, Rt
• Applications: WB, IHC
• Clone: OTI5C10
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

HOXC10 Antibody (OTI5C10) - Azide and BSA Free Summary

• Immunogen: Human recombinant protein fragment corresponding to amino acids 1-262 of human HOXC10(NP_059105) produced in E.coli.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: HOXC10
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin
  • Western Blot 1:2000
• Theoretical MW: 37.9 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from PBS (pH 7.3) with 8% Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Immunogen affinity purified
• Reconstitution Instructions: we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Alternate Names for HOXC10 Antibody (OTI5C10) - Azide and BSA Free

• homeo box C10
• homeobox C10
• Homeobox protein Hox-3I
• homeobox protein Hox-C10
• homeoprotein C10
• HOX3I
• MGC5259

Background

HOXC10 is 342 amino acids long, weighing approximately 38 kDa, that functions as a sequence-specific transcription factor which focuses on the specific positional identities of cells on the anterior-posterior axis, which is all part of a developmental regulatory system. Current studies are being done on several diseases and disorders including squamous cell carcinoma, cervicitis, lung carcinoma, and neuronitis. HOXB8 has also been shown to have interactions with EGFR, CDC27, SUMO2, NUP205, and YWHAG.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: HOXC10