HOIP/RNF31 Antibody [Alexa Fluor® 405]

• Reactivity: Hu
• Applications: WB, IHC, ICC/IF
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Alexa Fluor 405

HOIP/RNF31 Antibody [Alexa Fluor® 405] Summary

• Specificity: Detects human HOIP/RNF31 in direct ELISAs and Western blots.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry
  • Immunohistochemistry
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for HOIP/RNF31 Antibody [Alexa Fluor® 405]

• EC 6.3.2.-
• FLJ10111
• FLJ23501
• HOIP
• MGC19975
• ring finger protein 31
• RNF31
• ZIBRA
• ZIBRAHOIL-1-interacting protein
• Zinc in-between-RING-finger ubiquitin-associated domain protein

Background

RNF31 (RING [Really INteresting Gene] Finger Protein 31; also HOIL-1-interacting protein/HOIP, and Zn in-between-RING-finger ubiquitin-associated domain protein/ZIBRA) is a cytoplasmic E3 ubiquitin-protein ligase that is found in breast epithelium and multiple cancer types. Although its predicted MW is 102 kDa, it runs anomalously at 95-135 kDa in SDS-Page. Ubiquitin (Ub) chains are typically thought of as 9 kDa additions to Lys residues of target molecules. The activity associated with Ub addition depends upon the location of the attachment, and the monomeric vs. polymeric nature of the chains. Ub can also be added to N-terminal Met residues by an intracellular complex called LUBAC (Linear Ub chain Assembly Complex). This complex is key to NF kappa B pathway activation. Following exposure of cells to cytokines, LUBAC ubiquitinates NEMO, which subsequently induces IKK beta phosphorylation, I kappa B alpha degradation, and NF kappa B translocation into the nucleus with gene activation. The LUBAC complex contains RNF31, HOIL-1L and sharpin, and it is now known that RNF31 is the catalyst for linear Ub chain formation. Human RNF31 is 1072 amino acids (aa) in length. It contains three consecutive RanBP2-type Zn finger domains (aa 299-438), a utilized phosphoryation site at Ser466, one UBA domain (aa 564-615), and two RING-type Zn finger domains (aa 699-747 and 860-909) with an intervening IBR-type Zn finger region (aa 779-841). There are at least two isoform variants, one that contains a 13 aa substitution for aa 1-164, and another that shows a deletion of aa 73-630 coupled to a deletion of aa 833-841. Human RNF31 shares 87% aa sequence identity with mouse RNF31.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.