HM74A/PUMA-G/GPR109A/NIACR1 Antibody (MM0329-6S24) - Azide and BSA Free

HM74A/PUMA-G/GPR109A/NIACR1 Antibody (MM0329-6S24) - Azide and BSA Free [NBP2-12358] - Lysate of human placenta tissue with rat anti-human hM74A.

• Reactivity: Hu
• Applications: WB, Flow, IHC, CyTOF-ready
• Clone: MM0329-6S24
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

HM74A/PUMA-G/GPR109A/NIACR1 Antibody (MM0329-6S24) - Azide and BSA Free Summary

• Immunogen: Human recombinant HM74A/PUMA-G/GPR109A/NIACR1
• Isotype: IgG2
• Clonality: Monoclonal
• Host: Rat
• Gene: HCAR2
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 1:50-1:200
  • Immunohistochemistry 1:50-100
  • Western Blot 1:500-1:1000
• Application Notes: This antibody is Cytof ready.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from a 0.2 um filtered solution in PBS. 0.025 mg size is provided in liquid form, PBS
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Protein G purified
• Reconstitution Instructions: Reconstitute with sterilized PBS to a final concentration of 0.5 mg/ml.

Alternate Names for HM74A/PUMA-G/GPR109A/NIACR1 Antibody (MM0329-6S24) - Azide and BSA Free

• GPR109A
• HCA2
• HCAR2
• HM74A
• hydroxycarboxylic acid receptor 2
• Niacr1
• Nicotinic Acid Receptor
• PUMAG

Background

Nicotinic acid receptors are a group of Gi/Go protein-coupled receptors that are currently divided into GPR81, GPR109Aand GPR109B subtypes. GPR109B receptors originated from a gene duplication of GPR109A and have > 95% homology. Allsubtypes are widely expressed throughout adipose tissue and are found at lower levels on macrophages, neutrophils,Langerhans cells and in the spleen. Nicotinic acid receptors are involved in the inhibition of lipolysis. The humangenes encoding these receptors are located on chromosome 12 (12q24.3)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for HM74A/PUMA-G/GPR109A/NIACR1 Antibody (NBP2-12358)(1)

Publication using NBP2-12358

• Gautam J, Banskota S, Shah S, Jee JG. 4-Hydroxynonenal-induced GPR109A activation elicits bipolar responses, Gai-mediated anti-inflammatory effects, and Gbgamma-mediated cell death. Br. J. Pharmacol. 2018-02-23 [PMID: 29473951] (Human)

Bioinformatics

• Gene Symbol: HCAR2
• Uniprot:
  • Q8TDS4.1(Human)