Histone H2AY/macroH2A.1 Antibody (CL5245) - BSA Free

Western Blot: Histone H2AY/macroH2A.1 Antibody (CL5245) [NBP2-61155] - analysis in human cell line HEL.

Immunocytochemistry/Immunofluorescence: Histone H2AY/macroH2A.1 Antibody (CL5245) [NBP2-61155] - Staining of CACO-2 cells using the Anti-H2AFY monoclonal antibody, showing specific staining in the nucleoplasm in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).

Immunohistochemistry-Paraffin: Histone H2AY/macroH2A.1 Antibody (CL5245) [NBP2-61155] - staining of human tonsil shows strong nuclear immunoreactivity in lymphoid cells.

Immunohistochemistry-Paraffin: Histone H2AY/macroH2A.1 Antibody (CL5245) [NBP2-61155] - staining of human kidney shows strong nuclear positivity in renal tubules cells.

Immunohistochemistry-Paraffin: Histone H2AY/macroH2A.1 Antibody (CL5245) [NBP2-61155] - staining of human cerebellum shows strong nuclear immunoreactivity in neuronal and glial cells.

Immunohistochemistry-Paraffin: Histone H2AY/macroH2A.1 Antibody (CL5245) [NBP2-61155] - staining of human fallopian tube shows strong nuclear positivity in glandular and stromal cells.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC
• Clone: CL5245
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free

Histone H2AY/macroH2A.1 Antibody (CL5245) - BSA Free Summary

• Immunogen: This antibody was developed using a synthetic peptide derived from O75367, with the exact immunogen sequence remaining proprietary.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: MACROH2A1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 2-10 ug/ml
  • Immunohistochemistry 1:2500 - 1:5000
  • Immunohistochemistry-Paraffin 1:2500 - 1:5000
  • Western Blot 1 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. For ICC/IF, PFA/Triton X-100 is recommended for fixation/permeabilization.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Protein A purified

Alternate Names for Histone H2AY/macroH2A.1 Antibody (CL5245) - BSA Free

• core histone macro-H2A.1
• H2A histone family, member Y
• H2A.y
• H2A/y
• H2AF12M
• H2AFJ
• H2AFY
• Histone H2A.y
• Histone H2AY
• Histone MacroH2A1
• histone macroH2A1.1
• histone macroH2A1.2
• MACROH2A1
• MACROH2A1.1
• macroH2A1.2
• Medulloblastoma Antigen MU-MB-50.205
• mH2A1

Background

macroH2A.1 (H2AFY) is 372 amino acids long, weighs approximately 40 kDa, and is part of a group of basic nuclear proteins called histones, which are responsible for nucleosome structure of the chromosomal fiber in eukaryotes. macroH2a.1 has two shorter isoforms meeasuring 371 and 369 amino acids long, both weighing approximately 39 kDa. Current studies are being done on several diseases and disorders including medulloblastoma, lupus erythematosus, Huntington's disease, immunodeficiency, melanoma, and malaria. macroH2A.1 has also been shown to have interactions with HIST1H4A, HIST1H4B, HIST1H4C, HIST1H4D, and HIST1H4E in pathways such as the chromatin regulation/ acetylation, systemic lupus erythematosus, and ATF-2 transcription factor pathways.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: MACROH2A1