Histone H2AY/macroH2A.1 Antibody (866416)

Western blot shows lysates of MCF-7 human breast cancer cell line, Hepa 1-6 mouse hepatoma cell line, and Rat-2 rat embryonic fibroblast cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human/Mouse/Rat Histone H2AY Monoclonal Antibody (Catalog # MAB8318) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Histone H2AY at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Histone H2AY was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Mouse Anti-Human/Mouse/Rat Histone H2AY Monoclonal Antibody (Catalog # MAB8318) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (yellow, upper panel; Catalog # NL007) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF
• Clone: 866416
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

Histone H2AY/macroH2A.1 Antibody (866416) Summary

• Immunogen: E. coli-derived recombinant human Histone H2AY
  Gly230-Thr348
  Accession # O75367
• Specificity: Detects human Histone H2AY in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: H2AFY
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 8-25 ug/mL
  • Western Blot 2 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Histone H2AY/macroH2A.1 Antibody (866416)

• core histone macro-H2A.1
• H2A histone family, member Y
• H2A.y
• H2A/y
• H2AF12M
• H2AFJ
• H2AFY
• Histone H2A.y
• Histone H2AY
• Histone MacroH2A1
• histone macroH2A1.1
• histone macroH2A1.2
• MACROH2A1
• MACROH2A1.1
• macroH2A1.2
• Medulloblastoma Antigen MU-MB-50.205
• mH2A1

Background

Histone H2AY, also known as macro H2A1 (MH2A1) and H2AFY, is a 40 kDa variant of the histone family, basic nuclear proteins responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Ubiquitously expressed, H2AY replaces conventional H2A histones in a subset of nucleosomes, where it represses transcription and participates in stable X chromosome inactivation. H2AY expression is also enriched in senescence-associated heterochromatin. Alternative splicing creates multiple transcript variants, encoding three distinct isoforms. H2AY expressed in blood has been identified as a potential biomarker associated with the activity and therapeutic response of Huntington disease, an autosomal dominant neurodegenerative disorder. Over amino acids 230-348, human H2AY shares 98% identity with mouse and rat H2AY.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Histone H2AY/macroH2A.1 Antibody (MAB8318)(3)

Publications using MAB8318

• Guo Y, Chomiak AA, Hong Y et al. Histone H2A ubiquitination resulting from Brap loss of function connects multiple aging hallmarks and accelerates neurodegeneration iScience 7/15/2022 [PMID: 35754718]
• Chomiak AA, Guo Y, Kopsidas CA et al. Nde1 is required for heterochromatin compaction and stability in neocortical neurons iScience 2022-06-17 [PMID: 35601919]
• Chomiak AA, Guo Y, Kopsidas CA et al. Nde1 is required for heterochromatin compaction and stability in neocortical neurons iScience 2022-06-17 [PMID: 35601919]

Bioinformatics

• Gene Symbol: H2AFY
• Uniprot:
  • O75367()