Histone H2AX [p Ser139] Antibody (EP854(2)Y)

Product Discontinued

Histone H2AX [p Ser139] Antibody (EP854(2)Y) Summary

• Immunogen: A synthetic phospho-peptide corresponding to residues surrounding serine 139 of human H2A.x protein.
• Modification: p Ser139
• Marker: DNA Double-strand break marker
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: H2AX
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Chromatin Immunoprecipitation 1:10-1:500
  • Flow Cytometry 1:100
  • Immunocytochemistry/ Immunofluorescence 1:50-100
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin 1:50-100
  • Immunoprecipitation 1:40
  • Western Blot 1:1000-10000
• Application Notes: In research publications, this product has been cited for ChIP (PMID: 20360682) and Immunocytochemistry/Immunofluorescence (PMID: 22278880) applications Use in Immunohistochemistry-Frozen reported in scientific literature (PMID 24509083). Use in Immunoprecipition reported in scientific literature (PMID 24449872).
• Theoretical MW: 15 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: 59% PBS (pH 7.2), 0.05% BSA and 40% Glycerol
• Preservative: 0.01% Sodium Azide
• Purity: Protein A purified

Notes

Licensed to Novus Biologicals LLC under U.S. Patent Nos. 6,362,317 and 6,884,873. Manufactured incorporating RabMab® technology under Epitomics US patents, No 5675,063 and 7,429,487, owned by Abcam.

Alternate Names for Histone H2AX [p Ser139] Antibody (EP854(2)Y)

• H2A.X
• H2A/X
• H2AFX
• Histone H2AX

Background

Gamma H2AX (gammaH2AX) is the phosphorylated version of histone H2AX and is a marker for double-stranded breaks (DSBs) caused by DNA damage (1-4). H2AX is a variant of histone H2A, one of the histone core molecules forming the nucleosome, and is a vital component in repairing DNA damage (1-4). The H2AX variant represents between 2-25% of total H2A (1-3). Human H2AX protein is 143 amino acids in length and has a theoretical molecular weight of 15 kDa (5). As a result of DSBs, H2AX becomes phosphorylated at serine-139 and gamma H2AX is generated as a response to genomic instability (1-4). Gamma H2AX and its phosphorylation can be detected through western blotting and immunofluorescence, serving as a biomarker for DSB frequency (1-4). Ataxia telangiectasia mutated (ATM) is considered a key protein for H2AX phosphorylation (1-4). Upon the phosphorylation and formation of gamma H2AX, many repair proteins become associated including breast cancer 1 protein (BRCA1), NBS1 (Nijmegen breakage syndrome 1)/hMRE11 (human meiotic recombination 11 protein)/ hRAD50 (N/M/R) repair complex, and 53BP1 (p53 binding protein 1) (1-4). Gamma H2AX detection assays are currently utilized for a variety of purposes, from studying DNA repair to drug development and radiation biodosimetry (4).

References

1. Palla, V. V., Karaolanis, G., Katafigiotis, I., Anastasiou, I., Patapis, P., Dimitroulis, D., & Perrea, D. (2017). gamma-H2AX: Can it be established as a classical cancer prognostic factor?. Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine. https://doi.org/10.1177/1010428317695931

2. Kuo, L. J., & Yang, L. X. (2008). Gamma-H2AX - a novel biomarker for DNA double-strand breaks. In vivo (Athens, Greece).

3. Kinner, A., Wu, W., Staudt, C., & Iliakis, G. (2008). Gamma-H2AX in recognition and signaling of DNA double-strand breaks in the context of chromatin. Nucleic acids research. https://doi.org/10.1093/nar/gkn550

4. Redon, C. E., Weyemi, U., Parekh, P. R., Huang, D., Burrell, A. S., & Bonner, W. M. (2012). gamma-H2AX and other histone post-translational modifications in the clinic. Biochimica et biophysica acta. https://doi.org/10.1016/j.bbagrm.2012.02.021

5. H2AX: Uniprot (P16104)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for gamma H2AX Antibody (NB100-79967)(23)

Publications using NB100-79967

• Torres-Querol C, Torres P, Vidal N Et al. Acute ischemic stroke triggers a cellular senescence-associated secretory phenotype Scientific reports 2021-08-03 [PMID: 34344977] (IHC-Fr, Mouse)
• Kim DE, DollE MET, Vermeij WP et al. Deficiency in the DNA repair protein ERCC1 triggers a link between senescence and apoptosis in human fibroblasts and mouse skin Aging Cell 2019-11-18 [PMID: 31737985]
• Nakanuma Y, Kakuda Y, Uesaka K et al. Characterization of intraductal papillary neoplasm of bile duct with respect to histopathological similarities to pancreatic intraductal papillary mucinous neoplasm. Gut Liver 2019-04-15 [PMID: 30982236]
• Wang BY, Liao ML, Hong GC et al. Near-Infrared-Triggered Photodynamic Therapy toward Breast Cancer Cells Using Dendrimer-Functionalized Upconversion Nanoparticles Nanomaterials (Basel) 2017-09-11 [PMID: 28892021] (IHC-P, Mouse)
• Dai MS, Hall SJ, Vantangoli Policelli MM et al. Spontaneous testicular atrophy occurs despite normal spermatogonial proliferation in a Tp53 knockout rat Andrology 2017-08-22 [PMID: 28834365] (IHC-P, Rat)
• Lu T, Zhang Y, Kidane Y et al. Cellular responses and gene expression profile changes due to bleomycin-induced DNA damage in human fibroblasts in space. PLoS ONE. 2017-03-01 [PMID: 28248986] (ICC/IF, Human)
• Liu X, Liu F, Gao S et al. A single non-synonymous NCOA5 variation in type 2 diabetic patients with hepatocellular carcinoma impairs the function of NCOA5. Cancer Letters 2017-01-27 [PMID: 28137631] (ICC/IF, Human)
• Nikitaki Z, Nikolov V, Mavragani IV et al. Measurement of complex DNA damage induction and repair in human cellular systems after exposure to ionizing radiations of varying linear energy transfer (LET). Free Radic Res 2016-11-01 [PMID: 27593437] (ICC/IF, Human)
• Sigl V, Owusu-Boaitey K, Joshi PA et al. RANKL/RANK control Brca1 mutation-driven mammary tumors. Cell Res. 2016-07-01 [PMID: 27241552] (IF/IHC, Mouse)
• Zhang X, Wojtkowiak JW, Martinez GV et al. MR Imaging Biomarkers to Monitor Early Response to Hypoxia-Activated Prodrug TH-302 in Pancreatic Cancer Xenografts. PLoS ONE. 2016-05-27 [PMID: 27227903] (IHC-P, Mouse)
• Magimaidas A, Madireddi P, Maifrede S et al. Gadd45b deficiency promotes premature senescence and skin aging. Oncotarget. 2016-05-10 [PMID: 27105496] (Mouse)
• Kinoshita M, Kubo S, Nakanuma Y et al. Pathological spectrum of bile duct lesions from chronic bile duct injury to invasive cholangiocarcinoma corresponding to bile duct imaging findings of occupational cholangiocarcinoma. J Hepatobiliary Pancreat Sci. 2015-11-18 [PMID: 26580863]
• Thuy LT, Matsumoto Y, Thuy TT et al. Cytoglobin Deficiency Promotes Liver Cancer Development from Hepatosteatosis through Activation of the Oxidative Stress Pathway Am. J. Pathol. 2015-02-07 [PMID: 25665792] (IHC-P, Mouse, Human)
• Sato Y, Kubo S, Takemura S et al. Different carcinogenic process in cholangiocarcinoma cases epidemically developing among workers of a printing company in Japan Int J Clin Exp Pathol 2014-09-09 [PMID: 25197345]
• Qiu H, Lee S, Shang Y et al. ALS-associated mutation FUS-R521C causes DNA damage and RNA splicing defects. J Clin Invest 2014-03-01 [PMID: 24509083] (IHC-Fr, Mouse)
• Rao S, Tortola L, Perlot T et al. A dual role for autophagy in a murine model of lung cancer. Nat Commun. 2014-01-01 [PMID: 24445999] (IHC-P, Mouse)
• Taniai E, Yafune A, Nakajima M et al. Ochratoxin A induces karyomegaly and cell cycle aberrations in renal tubular cells without relation to induction of oxidative stress responses in rats. Toxicol Lett. 2013-10-11 [PMID: 24120684] (IHC-P, Rat)
• Fung C, Pupo GM, Scolyer RA et al. p16(INK) (4a) deficiency promotes DNA hyper-replication and genetic instability in melanocytes. Pigment Cell Melanoma Res. 2013-03-01 [PMID: 23279822] (ICC/IF, Human)
• Taniai Eriko, Hayashi Hitomi, Yafune Atsunori et al. Cellular distribution of cell cycle-related molecules in the renal tubules of rats treated with renal carcinogens for 28 days: relationship between cell cycle aberration and carcinogenesis. Arch Toxicol. 2012-09-01 [PMID: 22411272] (IF/IHC, Rat)
• Velarde MC, Flynn JM, Day NU et al. Mitochondrial oxidative stress caused by Sod2 deficiency promotes cellular senescence and aging phenotypes in the skin. Aging. 2012-01-20 [PMID: 22278880]
• J,,maa S, Af H,llstrm TM, Sankila A et al. DNA damage recognition via activated ATM and p53 pathway in nonproliferating human prostate tissue. Cancer Res 2010-11-01 [PMID: 20978201]
• Iacovoni JS, Caron P, Lassadi I et al. High-resolution profiling of gammaH2AX around DNA double strand breaks in the mammalian genome. EMBO J 2010-04-01 [PMID: 20360682] (ICC/IF, Chemotaxis)
• Schmid U, Stopper H, Schweda F et al. Angiotensin II induces DNA damage in the kidney. Cancer Res 2008-11-01 [PMID: 19010896] (ICC/IF)

Review for gamma H2AX Antibody (NB100-79967) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Verified Customer IF Human 01/22/2016

Summary

• Application: Immunofluorescence
• Species: Human
• Lot: Y1072211DS3

FAQs for gamma H2AX Antibody (NB100-79967). (Showing 1 - 1 of 1 FAQs).

1. What is the concentration of product NB100-79967 (lot: GR93354-9 & lot: GR93354-11) ?
   • Lots GR93354-9 and GR93354-11 of our gamma H2AX (pSer139) antibody with catalogue number NB100-79967 are both supplied at a concentration of 0.048mg/ml.

Bioinformatics

• Gene Symbol: H2AX
• Entrez:
  • 3014(Human)
  • 15270(Mouse)
  • 500987(Rat)
• Uniprot:
  • P16104()