Histone H1.4 [p Thr17] Antibody (SR1513) - BSA Free

Immunohistochemistry: Histone H1.4 [p Thr 17] Antibody (SR1513) [NBP3-21528] - Immunohistochemical analysis of paraffin-embedded mouse colon, using NBP3-21528.

Western Blot: Histone H1.4 [p Thr 17] Antibody (SR1513) [NBP3-21528] - Western blot analysis using NBP3-21528 in Jurkat cell lysate.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC
• Clone: SR1513
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

Histone H1.4 [p Thr17] Antibody (SR1513) - BSA Free Summary

• Description: Novus Biologicals Rabbit Histone H1.4 [p Thr17] Antibody (SR1513) - BSA Free (NBP3-21528) is a recombinant monoclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: A synthesized peptide derived from human phospho-Histone H1.4 [Thr17] (Uniprot #: P10412)
• Modification: p Thr17
• Specificity: Detects endogenous levels of total phospho-Histone H1.4 [Thr17]
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: H1-4
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry 1:50-1:200
  • Western Blot 1:500-1:2000
• Theoretical MW: 30 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS, pH 7.4, 150mM NaCl, 50% glycerol.
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for Histone H1.4 [p Thr17] Antibody (SR1513) - BSA Free

• dJ221C16.5
• H1 histone family, member 4
• H1.4
• H1F4H1E
• histone 1, H1e
• histone cluster 1, H1e
• histone H1.4
• Histone H1b
• MGC116819

Background

The HIST1H1E gene encodes a 219 amino acid long, 21 kDA Histone H1.4 that functions by binding to linker DNA between nucleosome creating the macromolecular structure chromatin fiber. H1 histones as especially critical in condensation of nucleosome chains and the monitoring of individual gene transcription. HIST1H1E participates in PKA signaling, cell cycle start of DNA replication in early S phase, apoptosis, and histone modification. It interacts with genes HIST1H4A, HIST1H4B, HIST1H4C, HIST1H4D, and HIST1H4L. HIST1H1E has been researched regarding its role in progressive supranuclear palsy, pancreatitis, hepatitis, and x inactivation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: H1-4