Histone H1 Antibody - BSA Free

Immunocytochemistry/Immunofluorescence: Histone H1 Antibody [NBP3-17658] - Staining of human cell line U-2 OS shows localization to nucleoplasm.

Immunohistochemistry-Paraffin: Histone H1 Antibody [NBP3-17658] - Staining of human testis shows strong nuclear positivity in cells in seminiferous ducts.

• Reactivity: Hu
• Applications: ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

Histone H1 Antibody - BSA Free Summary

• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: PAKAAAASKKKPAGPSVSELIVQAASSSKERG
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: H1-1
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:500 - 1:1000
  • Immunohistochemistry-Paraffin 1:500 - 1:1000
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence, PFA/Triton X-100 is recommended for fixation/permeabilization.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS, pH 7.2, 40% glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for Histone H1 Antibody - BSA Free

• H1 histone family, member 1
• H1.1
• H1A
• H1F1HIST1
• histone 1, H1a
• histone cluster 1, H1a
• histone H1.1
• MGC126642
• MGC138345

Background

Histone H1 is the linker protein that is 194 amino acids long, weights approximately 21 kDa, and is part of a group of basic nuclear proteins called histones, which are responsible for nucleosome structure of the chromosomal fiber in eukaryotes. Histone H1 has a shorter isoform measuring 177 amino acids long and weighing approximately 19 kDa as well. Current studies are being done on several diseases and disorders including paratyphoid fever, hemochromatosis, breast cancer, embryonal carcinoma, prostatitis, carcinoma, and neuronitis. This protein has also been shown to have interactions with ADPRH, CDK2, CDK7, CTCFL, and DOT1L in pathways such as the histone modification, DNA replication, chromosome condensation, PKA signaling, granzyme-A, and apoptosis pathways.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: H1-1