HIST1H3A Antibody (CL4967)

Images

 
Western Blot: HIST1H3A Antibody (CL4967) [NBP2-61150] - Western blot analysis of purified human recombinant histone H3, CenH3, H3.3, H2A, H2B and H4.
Immunohistochemistry: HIST1H3A Antibody (CL4967) [NBP2-61150] - Immunohistochemical staining of human kidney shows strong nuclear positivity in renal tubules cells.
Western Blot: HIST1H3A Antibody (CL4967) [NBP2-61150] - Western blot analysis in human cell line HeLa.
Western Blot: HIST1H3A Antibody (CL4967) [NBP2-61150] - Western blot analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.
Immunohistochemistry: HIST1H3A Antibody (CL4967) [NBP2-61150] - Immunohistochemical staining of human testis shows strong nuclear positivity in cells in seminiferous tubules.
Immunohistochemistry: HIST1H3A Antibody (CL4967) [NBP2-61150] - Immunohistochemical staining of human endometrium shows strong nuclear positivity in glandular and stromal cells.
Immunohistochemistry: HIST1H3A Antibody (CL4967) [NBP2-61150] - Immunohistochemical staining of human cerebellum shows strong nuclear immunoreactivity in neuronal and glial cells.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, IHC, IHC-P
Clone
CL4967
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

HIST1H3A Antibody (CL4967) Summary

Immunogen
This antibody was developed against a synthetic peptide corresponding to the following amino acid sequence:PKDIQLARRIRGERA
Specificity
Specificity of human, mouse, rat HIST1H3A antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
HIST1H3A
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/ml
  • Immunohistochemistry 1:10000 - 1:20000
  • Immunohistochemistry-Paraffin 1:10000 - 1:20000
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Protein A purified

Alternate Names for HIST1H3A Antibody (CL4967)

  • H3 histone family, member A
  • H3/A
  • H3FAHIST1H3E
  • H3FB
  • H3FC
  • H3FD
  • H3FF
  • H3FH
  • H3FI
  • H3FJ
  • H3FK
  • H3FL
  • HIST1H3B
  • HIST1H3C
  • HIST1H3D
  • HIST1H3F
  • HIST1H3G
  • HIST1H3H
  • HIST1H3I
  • HIST1H3J
  • histone 1, H3a
  • histone cluster 1, H3a
  • histone H3.1
  • Histone H3/a
  • Histone H3/b
  • Histone H3/c
  • Histone H3/d
  • Histone H3/f
  • Histone H3/h
  • Histone H3/i
  • Histone H3/j
  • Histone H3/k
  • Histone H3/l

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol HIST1H3A