![Western Blot: HIST1H3A Antibody (CL4967) [NBP2-61150] - Western blot analysis of purified human recombinant histone H3, CenH3, H3.3, H2A, H2B and H4.](http://images.novusbio.com/fullsize/HIST1H3A-Antibody-CL4967-Western-Blot-NBP2-61150-img0007.jpg "Western Blot: HIST1H3A Antibody (CL4967) [NBP2-61150] - Western blot analysis of purified human recombinant histone H3, CenH3, H3.3, H2A, H2B and H4.")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, ICC/IF, IHC |
| Clone | CL4967 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Description | Novus Biologicals Mouse HIST1H3A Antibody (CL4967) - BSA Free (NBP2-61150) is a monoclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee. |
| Immunogen | This antibody was developed using a synthetic peptide derived from P68431, with the exact immunogen sequence remaining proprietary. |
| Isotype | IgG2a |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | H3C1 |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | For IHC-Paraffin, HIER pH 6 retrieval is recommended. For ICC/IF, PFA/Triton X-100 is recommended for fixation/permeabilization. |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7.2) and 40% Glycerol |
| Preservative | 0.02% Sodium Azide |
| Purity | Protein A purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for HIST1H3A Antibody (NBP2-61150)Find related products by research area.
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| Gene Symbol | H3C1 |
![Immunocytochemistry/Immunofluorescence: HIST1H3A Antibody (CL4967) [NBP2-61150] - Staining of U-2 OS cells using the Anti-HIST1H3A monoclonal antibody, showing specific staining in the nucleus in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).](http://images.novusbio.com/fullsize/HIST1H3A-Antibody-CL4967-Immunocytochemistry-Immunofluorescence-NBP2-61150-img0008.jpg "Immunocytochemistry/Immunofluorescence: HIST1H3A Antibody (CL4967) [NBP2-61150] - Staining of U-2 OS cells using the Anti-HIST1H3A monoclonal antibody, showing specific staining in the nucleus in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).")
![Immunohistochemistry-Paraffin: HIST1H3A Antibody (CL4967) [NBP2-61150] - Immunohistochemical staining of human kidney shows strong nuclear positivity in renal tubules cells.](http://images.novusbio.com/fullsize/HIST1H3A-Antibody-CL4967-Immunohistochemistry-NBP2-61150-img0004.jpg "Immunohistochemistry-Paraffin: HIST1H3A Antibody (CL4967) [NBP2-61150] - Immunohistochemical staining of human kidney shows strong nuclear positivity in renal tubules cells.")
![Western Blot: HIST1H3A Antibody (CL4967) [NBP2-61150] - Western blot analysis in human cell line HeLa.](http://images.novusbio.com/fullsize/HIST1H3A-Antibody-CL4967-Western-Blot-NBP2-61150-img0005.jpg "Western Blot: HIST1H3A Antibody (CL4967) [NBP2-61150] - Western blot analysis in human cell line HeLa.")
![Western Blot: HIST1H3A Antibody (CL4967) [NBP2-61150] - Western blot analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.](http://images.novusbio.com/fullsize/HIST1H3A-Antibody-CL4967-Western-Blot-NBP2-61150-img0006.jpg "Western Blot: HIST1H3A Antibody (CL4967) [NBP2-61150] - Western blot analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.")
![Immunohistochemistry-Paraffin: HIST1H3A Antibody (CL4967) [NBP2-61150] - Immunohistochemical staining of human testis shows strong nuclear positivity in cells in seminiferous tubules.](http://images.novusbio.com/fullsize/HIST1H3A-Antibody-CL4967-Immunohistochemistry-NBP2-61150-img0001.jpg "Immunohistochemistry-Paraffin: HIST1H3A Antibody (CL4967) [NBP2-61150] - Immunohistochemical staining of human testis shows strong nuclear positivity in cells in seminiferous tubules.")
![Immunohistochemistry-Paraffin: HIST1H3A Antibody (CL4967) [NBP2-61150] - Immunohistochemical staining of human endometrium shows strong nuclear positivity in glandular and stromal cells.](http://images.novusbio.com/fullsize/HIST1H3A-Antibody-CL4967-Immunohistochemistry-NBP2-61150-img0002.jpg "Immunohistochemistry-Paraffin: HIST1H3A Antibody (CL4967) [NBP2-61150] - Immunohistochemical staining of human endometrium shows strong nuclear positivity in glandular and stromal cells.")
![Immunohistochemistry-Paraffin: HIST1H3A Antibody (CL4967) [NBP2-61150] - Immunohistochemical staining of human cerebellum shows strong nuclear immunoreactivity in neuronal and glial cells.](http://images.novusbio.com/fullsize/HIST1H3A-Antibody-CL4967-Immunohistochemistry-NBP2-61150-img0003.jpg "Immunohistochemistry-Paraffin: HIST1H3A Antibody (CL4967) [NBP2-61150] - Immunohistochemical staining of human cerebellum shows strong nuclear immunoreactivity in neuronal and glial cells.")
