Hexim 1 Antibody [Alexa Fluor® 594]

• Reactivity: Hu
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Alexa Fluor 594

Hexim 1 Antibody [Alexa Fluor® 594] Summary

• Immunogen: E. coli-derived recombinant human Hexim 1
  His213-Asp359
  Accession # O94992
• Specificity: Detects human Hexim 1 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human Hexim 2 is observed.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Hexim 1 Antibody [Alexa Fluor® 594]

• Cardiac lineage protein 1
• CLP1
• CLP-1
• CLP1hexamethylene-bis-acetamide-inducible transcript 1
• EDG1HMBA-inducible
• Estrogen down-regulated gene 1 protein
• hexamethylene bis-acetamide inducible 1
• Hexamethylene bis-acetamide-inducible protein 1
• hexamthylene bis-acetamide inducible 1
• Hexim 1
• HEXIM1
• HIS1
• HIS1FLJ13562
• HMBA-inducible
• MAQ1
• MAQ1hexamethylene bisacetamide-inducible protein
• menage a quatre 1
• Menage a quatre protein 1
• protein HEXIM1

Background

Hexim 1, also known as CLP1, EDG1, HIS1, and MAQ1, is an approximately 60 kDa transcriptional regulator that binds double stranded RNA and inhibits RNA polymerase II. Hexim 1 plays an important role in many processes including satellite muscle cell regeneration, cardiovascular development, and HIV-1 replication. It can form homodimers and heterodimers with Hexim 2, and its activity is regulated by phosphoylation at Ser158. Hexim 1 inhibits the kinase activity of the elongation factor P-TEFb. This inhibition can be relieved by Hexim 1 interaction with Nucleophosphmin or by cellular exposure to hexamtehylene bisacetamide. Hexim 1 inhibits the transactivation function of the glucocorticoid, estrogen, and androgen receptors. It can inhibit the cellular response to hypoxia and promote cancer progression or, alternatively, can inhibit VEGF mediated angiogenesis and suppress metastasis. Within aa 213-359, human Hexim 1 shares 95% and 94% aa sequence identity with mouse and rat Hexim 1, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.