Hepassocin/FGL1 Antibody

Western Blot: Hepassocin/FGL1 Antibody [NBP2-16486] - Sample (50 ug of whole cell lysate) A: Mouse Liver, 10% SDS PAGE gel, diluted at 1:1000.

Immunohistochemistry-Paraffin: Hepassocin/FGL1 Antibody [NBP2-16486] - U87 xenograft. FGL1 antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Western Blot: Hepassocin/FGL1 Antibody [NBP2-16486] - Sample (30 ug of whole cell lysate) A: Hep G2 10% SDS PAGE gel, diluted at 1:1000.

Product Discontinued

Hepassocin/FGL1 Antibody Summary

• Immunogen: Recombinant protein encompassing a sequence within the center region of human FGL1. The exact sequence is proprietary.
• Localization: Secreted
• Predicted Species: Bovine (92%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: FGL1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 36 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Rat (86%), Mouse (86%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7), 20% Glycerol
• Preservative: 0.025% Proclin 300
• Purity: Immunogen affinity purified

Alternate Names for Hepassocin/FGL1 Antibody

• FGL1
• fibrinogen-like 1
• fibrinogen-like protein 1
• Fibrinogen-like-protein 1
• FREP1
• Hepassocin
• hepatocellular carcinoma-related sequence
• Hepatocyte-derived fibrinogen-related protein 1
• HFREP-1
• HFREP1MGC12455
• HP-041
• LFIRE1
• LFIRE-1
• Liver fibrinogen-related protein 1

Background

Fibrinogen-like 1 is a member of the fibrinogen family. This protein is homologous to the carboxy terminus of the fibrinogen beta- and gamma- subunits which contains the four conserved cysteines of fibrinogens and fibrinogen related proteins. However, this protein lacks the platelet-binding site, cross-linking region and a thrombin-sensitive site which are necessary for fibrin clot formation. This protein may play a role in the development of hepatocellular carcinomas. Four alternatively spliced transcript variants encoding the same protein exist for this gene. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: FGL1
• Uniprot:
  • Q08830()