HECTD2 Antibody - Azide and BSA Free

Western Blot: HECTD2 Antibody [H00143279-B01P] - Analysis of HECTD2 expression in transfected 293T cell line by HECTD2 polyclonal antibody. Lane 1: HECTD2 transfected lysate(22.77 KDa). Lane 2: Non-transfected lysate.

Immunocytochemistry/Immunofluorescence: HECTD2 Antibody [H00143279-B01P] - Analysis of purified antibody to HECTD2 on HeLa cell. (antibody concentration 10 ug/ml)

• Reactivity: Hu
• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

HECTD2 Antibody - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse HECTD2 Antibody - Azide and BSA Free (H00143279-B01P) is a polyclonal antibody validated for use in WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: HECTD2 (NP_775768.1, 1 a.a. - 207 a.a.) full-length human protein. MSEAVRVPSPATPLVVAAAAPEERKGKESEREKLPPIVSAGAGATAGLDRGAKGQISTFSSFISAVSPKKEAAENRSSPAHLVFPNIKNVREPPPICLDVRQKQRTSMDASSSEMKAPVLPEPILPIQPKTVKDFQEDVEKVKSSGDWKAVHDFYLTTFDSFPELNAAFKKDATASFNTIEDSGINAKFVNAVYDTLLNTVSIMTCK
• Specificity: HECTD2 - HECT domain containing 2,
• Isotype: IgG
• Clonality: Polyclonal
• Host: Mouse
• Gene: HECTD2
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Western Blot 1:500
• Application Notes: Antibody reactive against Recombinant Protein with GST tag on ELISA and Western Blot and also on transfected lysate in western blot. GST tag alone is used as a negative control. It has also been used for immunofluorescence.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4)
• Preservative: No Preservative
• Purity: Protein A purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for HECTD2 Antibody - Azide and BSA Free

• EC 6.3.2.-
• EC2.3.2.26
• FLJ16050
• FLJ37306
• HECT domain containing 2
• HECT domain-containing protein 2
• HECTD2
• probable E3 ubiquitin-protein ligase HECTD2

Background

Probable E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in theform of a thioester and then directly transfers the ubiquitin to targeted substrates

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: HECTD2
• Entrez:
  • 143279(Human)
• Uniprot:
  • NP_775768.1(Human)