![Western Blot: HECTD1 Antibody [NBP1-49926] - USP15 knockdown with three siRNAs in LN-229 and (b) two siRNAs in LN-428. Image collected and cropped by CiteAb from the following publication (//www.oncotarget.com/lookup/doi/10.18632/oncotarget.22798) licensed under a CC-BY license. USP15 antibody (catalog # NB110-40690)](http://images.novusbio.com/fullsize/HECTD1-Antibody-Western-Blot-NBP1-49926-img0003.jpg "Western Blot: HECTD1 Antibody [NBP1-49926] - USP15 knockdown with three siRNAs in LN-229 and (b) two siRNAs in LN-428. Image collected and cropped by CiteAb from the following publication (//www.oncotarget.com/lookup/doi/10.18632/oncotarget.22798) licensed under a CC-BY license. USP15 antibody (catalog # NB110-40690)")
| Reactivity | HuSpecies Glossary |
| Applications | IP, WB (-) |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Concentration | 1.0 mg/ml |
| Description | Novus Biologicals Rabbit HECTD1 Antibody - BSA Free (NBP1-49926) is a polyclonal antibody validated for use in WB and IP. Anti-HECTD1 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee. |
| Immunogen | The immunogen for this product maps to a region between residue 2100 and 2150 of human HECT Domain Containing 1 using the numbering given in entry AAP13073.1 (GeneID 25831). |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | HECTD1 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Storage | Store at 4C. Do not freeze. |
| Buffer | Tris-Citrate/Phosphate (pH 7.0 - 8.0) |
| Preservative | 0.09% Sodium Azide |
| Concentration | 1.0 mg/ml |
| Purity | Immunogen affinity purified |
| Publication using NBP1-49926 | Applications | Species |
|---|---|---|
| Oikonomaki M, Bady P, et al. Ubiquitin Specific Peptidase 15 (USP15) suppresses glioblastoma cell growth via stabilization of HECTD1 E3 ligase attenuating WNT pathway activity. Oncotarget 2017-12-15 [PMID: 29299163] (WB, Human) | WB | Human |
Secondary Antibodies |
Isotype Controls |
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![Immunoprecipitation: HECTD1 Antibody [NBP1-49926] - Detection of Human HECTD1 by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-HECTD1 antibody used for IP at 3 mcg/mg lysate. HECTD1 was also immunoprecipitated by rabbit anti-HECTD1 antibody which recognizes an upstream epitope. For blotting immunoprecipitated HECTD1 was used at 1 mcg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/HECTD1-Antibody-Immunoprecipitation-NBP1-49926-img0002.jpg "Immunoprecipitation: HECTD1 Antibody [NBP1-49926] - Detection of Human HECTD1 by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-HECTD1 antibody used for IP at 3 mcg/mg lysate. HECTD1 was also immunoprecipitated by rabbit anti-HECTD1 antibody which recognizes an upstream epitope. For blotting immunoprecipitated HECTD1 was used at 1 mcg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![Western Blot: HECTD1 Antibody [NBP1-49926] - USP15 interacts with HECTD1 in GBM cell linesImmunoprecipitation using an antibody against the endogenous USP15 in LN-229, LN-428, & LN-18 protein extracts, analyzed by Western blot using an antibody against HECTD1. The control immunoprecipitation was performed with anti-rabbit IgG. Image collected & cropped by CiteAb from the following publication (//www.oncotarget.com/lookup/doi/10.18632/oncotarget.22798), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-49926_rabbit-polyclonal-hectd1-antibody-31020241535696.jpg "Western Blot: HECTD1 Antibody [NBP1-49926] - USP15 interacts with HECTD1 in GBM cell linesImmunoprecipitation using an antibody against the endogenous USP15 in LN-229, LN-428, & LN-18 protein extracts, analyzed by Western blot using an antibody against HECTD1. The control immunoprecipitation was performed with anti-rabbit IgG. Image collected & cropped by CiteAb from the following publication (//www.oncotarget.com/lookup/doi/10.18632/oncotarget.22798), licensed under a CC-BY license. Not internally tested by Novus Biologicals.")
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")