H2A [ac Lys5] Antibody

Images

 
Dotblot analysis of anti-H2A antibody with peptide samples. Varied amount of peptide samples were spotted onto positively charged nylon membrane and blotted with H2A antibody (NBP3-48612) 1:5000 dilution.A: Peptide ...read more
Orthogonal Strategies: H2A antibody detects H2A protein by Western blot analysis.A. 30 ug HeLa whole cell lysate/extract (0.012% DMSO treatment for 18 hr)B. 30 ug HeLa whole cell lysate/extract (0.4 uM ...read more
H2A antibody immunoprecipitates H2A protein in IP experiments. IP Sample: HeLa whole cell lysate/extract A : 30 ug whole cell lysate/extract of H2A protein expressing HeLa cells (0.4uM TSA treatment for 18hr) B : ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, DB, ICC/IF, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
       

Orthogonal Strategies

 

Order Details

H2A [ac Lys5] Antibody Summary

Description
Novus Biologicals Rabbit H2A [ac Lys5] Antibody (NBP3-48612) is a polyclonal antibody validated for use in WB, ICC/IF and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
Immunogen
Carrier-protein conjugated synthetic peptide surrounding acetyl Lys5 of human H2A. The exact sequence is proprietary.
Modification
ac Lys5
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
HIST2H2AA3
Purity
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Dot Blot
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunoprecipitation 1:100-1:10000
  • Western Blot 1:1000-1:20000

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS, 1% BSA, 20% Glycerol
Preservative
0.01% Thimerosal
Purity
Antigen Affinity-purified

Alternate Names for H2A [ac Lys5] Antibody

  • H2A histone family, member O
  • H2A.2
  • H2A/O
  • H2A/q
  • H2a-615
  • H2AFOHistone H2A/o
  • HIST2H2AAH2A
  • histone 2, H2aa
  • histone 2, H2aa3
  • histone cluster 2, H2aa3
  • histone H2A type 2-A
  • Histone H2A.2

Background

H2A is 130 amino acids long, weighs approximately 14 kDa, and is part of a group of basic nuclear proteins called histones, which are responsible for nucleosome structure of the chromosomal fiber in eukaryotes. Current studies are being done on several diseases and disorders including systemic lupus erythematosus, lupus erythematosus, x inactivation, riddle syndrome, connective tissue disease, and immunodeficiency. H2A has also been shown to have interactions with BMI1, DNAJC2, RCC1, RNF2, and SRRM1 in pathways such as the telomere maintenance, nucleosome assembly, cell cycle, and systemic lupus erythematosus pathways.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for H2A Antibody (NBP3-48612) (0)

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Secondary Antibodies

 

Isotype Controls

Additional H2A Products

Array NBP3-48612

Blogs on H2A.

H3.1t - A testis-specific histone variant
Histones are nuclear proteins essential for the storage and organization of genomic DNA as chromatin. Chromatin consists of DNA wrapped tightly around histone oligomers to form nucleosomes. In addition to compacting the genome, histones also regula...  Read full blog post.

H3.1 - A core histone essential for genome storage and organization
Histones are the main protein component of chromatin and are essential for the storage and compaction of the genome. DNA wraps around histone oligomers to make up nucleosomes, the individual subunits of chromatin. By altering the accessibility of t...  Read full blog post.

Histone H3
Eukaryotic chromosomes are formed through the highly organized and structural wrapping of DNA genetic material around histone proteins into the classic "bead on a string" globular structure of nucleosomes. The histone family consists of five family me...  Read full blog post.

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Bioinformatics

Gene Symbol HIST2H2AA3