H1F0 Antibody (AE-4)

Images

 
Western Blot: H1F0 Antibody (AE-4) [NBP2-45182] - Histone H1 Antibody (AE-4) [NBP2-45182] - Western Blot Analysis of human heart tissue lysate using H1F0 antibody (AE-4)
Immunocytochemistry/ Immunofluorescence: H1F0 Antibody (AE-4) [NBP2-45182] - Immunofluorescence staining of PFA-fixed HeLa cells using H1F0 antibody (AE-4) followed by goat anti-mouse IgG-CF488 (green). Membrane stained ...read more
Immunohistochemistry-Paraffin: H1F0 Antibody (AE-4) [NBP2-45182] - Histone H1 Antibody (AE-4) [NBP2-45182] - Formalin-fixed, paraffin-embedded human Angiosarcoma stained with H1F0 antibody (AE-4)
Flow Cytometry: H1F0 Antibody (AE-4) [NBP2-45182] - Flow Cytometric Analysis of HeLa cells. H1F0 antibody (AE-4)followed by goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).
Immunohistochemistry-Paraffin: H1F0 Antibody (AE-4) [NBP2-45182] - Human Tonsil stained with H1F0 antibody (AE-4)
Immunohistochemistry-Paraffin: H1F0 Antibody (AE-4) [NBP2-45182] - Formalin-fixed, paraffin-embedded human Testicular Carcinoma stained with H1F0 antibody (AE-4)
Immunohistochemistry-Paraffin: H1F0 Antibody (AE-4) [NBP2-45182] - Formalin-fixed, paraffin-embedded human Pancreas stained with H1F0 antibody (AE-4)

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-P, ICC/IF
Clone
AE-4
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.2 mg/ml

Order Details

H1F0 Antibody (AE-4) Summary

Immunogen
Nuclei of human leukemia biopsy cells
Localization
Nuclear
Marker
Pan Nuclear Marker
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Mouse
Gene
H1-0
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 1-2 ug/million cells
  • Immunocytochemistry/Immunofluorescence 1-2 ug/ml
  • Immunofluorescence 0.5 - 1.0 ug/ml
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1-2 ug/ml
  • Western Blot 1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Theoretical MW
30 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C.
Buffer
1mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Purity
Protein A or G purified

Notes

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 1 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-47753)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C. Antibody is stable for 24 months. Non-hazardous.

Alternate Names for H1F0 Antibody (AE-4)

  • H1 histone family, member 0
  • H1.0, H1(0), H1-0
  • H10
  • H1FVMGC5241
  • Histone H1'
  • Histone H1(0)
  • histone H1.0

Background

Plasmid: pCMV-H1F0 Full length; Host Cell: 293T

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol H1-0
Uniprot