GTF3C4 Antibody - BSA Free

Western Blot: GTF3C4 Antibody [NBP2-57486] - Western blot analysis in human cell line RT-4 and human cell line U-251 MG.

Immunocytochemistry/Immunofluorescence: GTF3C4 Antibody [NBP2-57486] - Staining of human cell line HaCaT shows localization to nucleus.

ChIP-Exo-Seq composite graph for Anti-GTF3C4 (NBP2-57486) tested in K562 cells. Strand-specific reads (blue: forward, red: reverse) and IgG controls (black: forward, grey: reverse) are plotted against the distance from a composite set of reference binding sites. The antibody exhibits robust target enrichment compared to a non-specific IgG control and precisely reveals its structural organization around the binding site. Data generated by Prof. B. F. Pugh´s Lab at Cornell University.

• Reactivity: Hu
• Applications: WB, ICC/IF, ChIP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

GTF3C4 Antibody - BSA Free Summary

• Immunogen: This antibody was developed against a recombinant protein corresponding to the following amino acid sequence: FWRFKLFLLRILYQSMQKTPSEALWKPTHEDSKILLVDSPGMGNADDEQQEEGTSSKQVVKQGLQERSKEGDVEEPTDDSLPT
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: GTF3C4
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Chromatin Immunoprecipitation-exo-Seq 1-10ug per reaction
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Western Blot 0.04-0.4 ug/ml
• Application Notes: Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
• Control Peptide:

  GTF3C4 Recombinant Protein Antigen (NBP2-57486PEP)

Reactivity Notes

Mouse 82%, Rat 81%

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for GTF3C4 Antibody - BSA Free

• EC 2.3.1.48
• FLJ21002
• general transcription factor 3C 4
• general transcription factor 3C polypeptide 4
• general transcription factor IIIC, polypeptide 4 (90kD)
• general transcription factor IIIC, polypeptide 4, 90kDa
• KAT12
• TF3C-delta
• TFIII90
• TFIIIC 90 kDa subunit
• TFIIIC290
• TFIIIC90MGC138450
• TFIIICDELTA
• Transcription factor IIIC 90 kDa subunit
• Transcription factor IIIC subunit delta

Background

General transcription factor 3C polypeptide 4 (GTF3C4/TFIIIC90) is a subunit of the TFIIIC DNA-binding factor required for transcription of tRNA and 5S rRNA genes by RNA polymerase III. Human TFIIIC is composed of six subunits: TFIIIC220, TFIIIC110, TFIIIC102, TFIIIC90 and TFIIIC63, and TFIIIC35. GTF3C4/TFIIIC90 is required for RNA polymerase III transcription and interacts with TFIIIC220, TFIIIC110, TFCIIIC63, TFIIIB90, and RPC39. GTF3C4/TFIIIC90 has been shown to possess acetyltransferase activity specific for histone H3. Alternate names for GTF3C4/TFIIIC90 include transcription factor IIIC subunit delta, TF3C-delta, TFIIIC 90 kDa subunit, FLJ21002, and MGC138450.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: GTF3C4