GTF3C5 Antibody

Western Blot: GTF3C5 Antibody [NB100-60435] - Detection of human and mouse GTF3C5 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-GTF3C5 antibody NB100-60435 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.

Immunohistochemistry: GTF3C5 Antibody [NB100-60435] - Detection of human GTF3C5/TFIIIC63 by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti- GTF3C5/TFIIIC63 (NB100-60435). Detection: DAB

Western Blot: GTF3C5 Antibody [NB100-60435] - Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 ug), and mouse NIH3T3 (M; 50 ug) cells. NB100-60435 used for WB at 0.04 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate.

Immunohistochemistry-Paraffin: GTF3C5 Antibody [NB100-60435] - FFPE section of human thyroid carcinoma. Antibody dilution of 1:500.

Immunohistochemistry: GTF3C5 Antibody [NB100-60435] - Sample: FFPE section of human breast carcinoma (left) and mouse teratoma (right). Antibody: Affinity purified rabbit anti- GTF3C5/TFIIIC63 used at a dilution of 1:500 (0.4ug/ml). Detection: DAB

Immunohistochemistry: GTF3C5 Antibody [NB100-60435] - Detection of mouse GTF3C5/TFIIIC63 by immunohistochemistry. Sample: FFPE section of mouse teratoma. Antibody: Affinity purified rabbit anti- GTF3C5/TFIIIC63 (NB100-60435). Detection: DAB

Immunoprecipitation: GTF3C5 Antibody [NB100-60435] - Detection of human GTF3C5 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-GTF3C5 antibody NB100-60435 (lot NB100-60435-2) used for IP at 6 ug per reaction. GTF3C5 was also immunoprecipitated by a previous lot of this antibody (lot NB100-60435-1). For blotting immunoprecipitated GTF3C5, NB100-60435 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.

• Reactivity: Hu, Mu
• Applications: WB, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

GTF3C5 Antibody Summary

• Description: Novus Biologicals Rabbit GTF3C5 Antibody (NB100-60435) is a polyclonal antibody validated for use in IHC, WB and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: The immunogen recognized by this antibody maps to a region between residue 469 and 519 of human general transcription factor 3C polypeptide 5 (Transcription factor IIIC 63 kDa subunit) using the numbering given in entry NP_036219.1 (GeneID 9328).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: GTF3C5
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation 1:10-1:500
  • Western Blot 1:100-1:2000
• Control:

  GTF3C5 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: TBS and 0.1% BSA
• Preservative: 0.09% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for GTF3C5 Antibody

• FLJ20857
• general transcription factor 3C polypeptide 5
• general transcription factor IIIC, polypeptide 5 (63kD)
• general transcription factor IIIC, polypeptide 5, 63kDa
• TF3C-epsilon
• TFiiiC2-63
• TFIIIC63TFIIIC 63 kDa subunit
• TFIIICepsilon
• Transcription factor IIIC 63 kDa subunit
• Transcription factor IIIC subunit epsilon
• transcription factor IIIC, 63 kD

Background

General transcription factor 3C polypeptide 5 (GTF3C5/TFIIIC63) is a subunit of the TFIIIC DNA-binding factor required for transcription of tRNA and 5S rRNA genes by RNA polymerase III. Human TFIIIC is composed of six subunits: TFIIIC220, TFIIIC110, TFIIIC102, TFIIIC90 and TFIIIC63, and TFIIIC35. GTF3C5/TFIIIC63 interacts with TFIIIB and may facilitate TFIIIB and RNA polymerase III recruitment in association with TFIIIC102, TFIIIB90, and RPC62. Alternate names for GTF3C5/TFIIIC63 include transcription factor IIIC subunit epsilon, TF3C-epsilon, TFIIIC 63 kDa subunit, general transcription factor IIIC polypeptide 5 (63 kDa), and FLJ20857.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: GTF3C5
• Entrez:
  • 9328(Human)
  • 70239(Mouse)
• Uniprot:
  • NP_036219.1(Human)