Recombinant Human GSK-3 beta Protein

Product Discontinued

Recombinant Human GSK-3 beta Protein Summary

• Description: A recombinant protein corresponding to Human GSK-3 beta
• Preparation Method: >90% SDS-PAGE
• Details of Functionality: Specifications/Test method: Activity of GSK-3B was determined by an in vitro kinase assay using 100 ng Tau as substrate and 1 ug of the recombinant GSK-3B in a 20 ul reaction containing 25 mM Tris-HCl, pH 7.5, 5 mM B-glycerol phosphate, 12 mM MgCl2, 2 mM DTT, 0.1 M Na3VO4, and 200 uM ATP. After 30 minutes incubation at 30C, phosphorylation of Tau by the recombinant GSK-3B was determined by Western blot using a phospho-Tau antibody.
• Protein/Peptide Type: Recombinant Protein
• Gene: GSK3B
• Purity: >90% SDS-PAGE

Applications/Dilutions

• Theoretical MW: 54.6 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -80C. Avoid freeze-thaw cycles.
• Buffer: From 2 in 0.1 M NH4CO3.
• Concentration: LYOPH
• Purity: >90% SDS-PAGE
• Reconstitution Instructions: Reconstitute with PBS containing 15% glycerol to a final concentration of 1.0 mg/ml. The solution should be stored at -70C, stable for up to 3 months.

Alternate Names for Recombinant Human GSK-3 beta Protein

• EC 2.7.11
• EC 2.7.11.26
• glycogen synthase kinase 3 beta
• glycogen synthase kinase-3 beta
• GSK3 beta
• GSK-3 beta
• GSK3B
• GSK3beta isoform

Background

Glycogen synthase kinase-3 (GSK-3) is a serine/theronine kinase that was first characterized by its role in intermediary metabolism. In mammals, serine kinase is encoded by two related genes called GSK-3alpha (51 kDa) and GSK-3Beta (46 kDa). GSK-3 has been implicated in the regulation of the c-Jun/AP-1 transcription factor and other intracellular signaling pathways.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: GSK3B