Recombinant Human GSK-3 beta Protein

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SDS-Page: Recombinant Human GSK-3 beta Protein [NBP1-99842]

Product Details

Summary
Reactivity HuSpecies Glossary
Applications PAGE
Concentration
Lyoph

Order Details

Recombinant Human GSK-3 beta Protein Summary

Description
Recombinant protein for Human GSK-3 beta

Source:E. coli.
Amino Acid Sequence:(P49841)

Source
E. coli
Protein/Peptide Type
Biologically Active Protein
Gene
GSK3B
Purity
>90%, by SDS-PAGE

Applications/Dilutions

Application Notes
Specific activity: Activity of GSK-3B was determined by an in vitro kinase assay using 100 ng Tau as substrate and 1 ug of the recombinant GSK-3B in a 20 ul reaction containing 25 mM Tris-HCl, pH 7.5, 5 mM B-glycerol phosphate, 12 mM MgCl2, 2 mM DTT, 0.1 M Na3VO4, and 200 uM ATP. After 30 minutes incubation at 30C, phosphorylation of Tau by the recombinant GSK-3B was determined by Western blot using a phospho-Tau antibody.
Theoretical MW
54.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.
Buffer
Lyophilized in 0.1 M NH4CO3
Concentration
Lyoph
Purity
>90%, by SDS-PAGE
Reconstitution Instructions
Reconstitute to 1 mg/ml in PBS containing 15% glycerol. The solution should be stored at -70C, stable for up to 3 months.

Alternate Names for Recombinant Human GSK-3 beta Protein

  • EC 2.7.11
  • EC 2.7.11.26
  • glycogen synthase kinase 3 beta
  • glycogen synthase kinase-3 beta
  • GSK3 beta
  • GSK-3 beta
  • GSK3B
  • GSK3beta isoform

Background

Glycogen synthase kinase-3 (GSK-3) is a serine/theronine kinase that was first characterized by its role in intermediary metabolism. In mammals, serine kinase is encoded by two related genes called GSK-3alpha (51 kDa) and GSK-3Beta (46 kDa). GSK-3 has been implicated in the regulation of the c-Jun/AP-1 transcription factor and other intracellular signaling pathways.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 2 years from date of receipt.

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Bioinformatics

Gene Symbol GSK3B
Entrez