GSK-3 beta Antibody (SY28-03)

Western Blot: GSK-3 beta Antibody (SY28-03) [NBP2-67448] - Analysis of GSK-3 beta on different lysates with Rabbit anti-GSK-3 beta antibody at 1/500 dilution. Lane 1: PC-12 cell lysate Lane 2: Hela cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 47 kDa Observed band size: 40 kDa Exposure time: 2 minutes; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.

Immunocytochemistry/Immunofluorescence: GSK-3 beta Antibody (SY28-03) [NBP2-67448] - Staining GSK3 beta in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: GSK-3 beta Antibody (SY28-03) [NBP2-67448] - Analysis of paraffin-embedded mouse testis tissue using anti-GSK3 beta antibody. Counter stained with hematoxylin.

Flow Cytometry: GSK-3 beta Antibody (SY28-03) [NBP2-67448] - Analysis of Hela cells with GSK3 beta antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.

Western Blot: GSK-3 beta Antibody (SY28-03) [NBP2-67448] - Analysis of GSK3 beta on different lysates using anti-GSK3 beta antibody at 1/1,000 dilution. Positive control: Lane 1: Hela Lane 2: 293 Lane 3: NIH/3T3 Lane 4: PC12

Immunocytochemistry/Immunofluorescence: GSK-3 beta Antibody (SY28-03) [NBP2-67448] - Staining GSK3 beta in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunocytochemistry/Immunofluorescence: GSK-3 beta Antibody (SY28-03) [NBP2-67448] - Staining GSK3 beta in SKOV-3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: GSK-3 beta Antibody (SY28-03) [NBP2-67448] - Analysis of paraffin-embedded human breast carcinoma tissue using anti-GSK3 beta antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: GSK-3 beta Antibody (SY28-03) [NBP2-67448] - Analysis of paraffin-embedded human breast tissue using anti-GSK3 beta antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: GSK-3 beta Antibody (SY28-03) [NBP2-67448] - Analysis of paraffin-embedded mouse prostate tissue using anti-GSK3 beta antibody. Counter stained with hematoxylin.

Product Discontinued

GSK-3 beta Antibody (SY28-03) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Synthetic peptide within human GSK-3 beta aa 360-400. (SwissProt: P49841 Human; SwissProt: Q9WV60 Mouse; SwissProt: P18266 Rat)
• Localization: Cytoplasm, Nucleus, Cell membrane.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: GSK3B
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:100
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:500-1:2000
• Theoretical MW: 47 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for GSK-3 beta Antibody (SY28-03)

• EC 2.7.11
• EC 2.7.11.26
• glycogen synthase kinase 3 beta
• glycogen synthase kinase-3 beta
• GSK3 beta
• GSK-3 beta
• GSK3B
• GSK3beta isoform

Background

Glycogen synthase kinase 3beta (GSK-3 beta) is a Serine/Threonine protein kinas involved in the insulin and wingless pathways (1-2). Originally identified as a regulator of glycogen synthase, it has been shown to regulate a diverse array of cellular functions. GSK-3 beta activity is down regulated by phosphorylation on Serine 9 by PKB, or activated by phosphorylation on Tyrosine 216. Once activated, the protein can phosphorylate p53, c-jun, heat shock factor-1 and cyclin D1 (3-4). It is also known to phosphorylate Tau in Allzheimer's disease. Additionally, increased GSK-3 beta protein levels are found in Alzheimer's disease brains.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: GSK3B