GRK2 Antibody (3F8)

Immunohistochemistry: GRK2 Antibody (3F8) [NBP2-37611] - Localization of G protein-coupled receptor kinase 2 (GRK2) in two patients with takotsubo syndrome who underwent endomyocardial biopsy in both the acute and recovery phases. Micrographs showing immunofluorescence stainings of GRK2 in the acute and recovery phases. Arrowheads (yellow) indicate positive signals for GRK2. Image collected and cropped by CiteAb from the following publication (nature.com/articles/s41598-018-31034-z) licensed under a CC-BY license.

Western Blot: GRK2 Antibody (3F8) [NBP2-37611] - Analysis using GRK2 mouse mAb against Hela (1), Jurkat (2), MOLT4 (3), RAJI (4), THP-1 (5), L1210 (6), Cos7 (7), PC-12 (8), and NIH/3T3 (9) cell lysate.

Immunocytochemistry/Immunofluorescence: GRK2 Antibody (3F8) [NBP2-37611] - Analysis of NIH/3T3 cells using GRK2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.

Immunohistochemistry: GRK2 Antibody (3F8) [NBP2-37611] - Localization of G protein-coupled receptor kinase 2 (GRK2). (a) Immunohistofluorescence staining for GRK2 using the specific antibody. GRK2 (red) in the takotsubo syndrome (TTS) and dilated cardiomyopathy (DCM) groups were observed not only in the cytoplasm but also on the cell membrane, which was confirmed by colocalization with wheat germ agglutinin (WGA) (green) (Arrowheads). Blue staining localized to 4',6-diamidino-2-phenylindole (DAPI). Image collected and cropped by CiteAb from the following publication (nature.com/articles/s41598-018-31034-z) licensed under a CC-BY license.

Immunohistochemistry-Paraffin: GRK2 Antibody (3F8) [NBP2-37611] - Analysis of endometrial cancer tissues (left) and cervical cancer tissues (right) using GRK2 mouse mAb with DAB staining.

ELISA: GRK2 Antibody (3F8) [NBP2-37611] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);

Immunocytochemistry/ Immunofluorescence: GRK2 Antibody (3F8) - BSA Free [NBP2-37611] - Localization of G protein-coupled receptor kinase 2 (GRK2), beta -arrestin2, & 8-hydroxy-2′deoxyguanosine (8-OHdG) in two patients with takotsubo syndrome who underwent endomyocardial biopsy in both the acute & recovery phases. Micrographs showing immunofluorescence stainings of GRK2 & beta -arrestin2, & immunohistochemical staining of 8-OHdG in the acute & recovery phases. Arrowheads (yellow) indicate positive signals for GRK2 & beta -arrestin2 on the cell membrane, & Arrowheads (red) indicate 8-OHdG-positive nuclei. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30143703), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunocytochemistry/ Immunofluorescence: GRK2 Antibody (3F8) - BSA Free [NBP2-37611] - Localization of G protein-coupled receptor kinase 2 (GRK2). (a) Immunohistofluorescence staining for GRK2 using the specific antibody. GRK2 (red) in the takotsubo syndrome (TTS) & dilated cardiomyopathy (DCM) groups were observed not only in the cytoplasm but also on the cell membrane, which was confirmed by colocalization with wheat germ agglutinin (WGA) (green) (Arrowheads). Blue staining localized to 4′,6-diamidino-2-phenylindole (DAPI). (b) Quantification for GRK2 positive stained area in the myocardium. The box represents the 25th & 75th percentiles & the line the median value. Whiskers correspond to the 25th percentile minus 1.5 times interquartile range (IQR) & to the 75th percentile plus 1.5 IQR. (c) Quantification for membranous GRK2 positive cardiomyocyte. *P < 0.001 vs. the normal control (NC) group. †P < 0.001 vs. the DCM group. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30143703), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

• Reactivity: Hu, Mu, Rt, Pm, Pm
• Applications: WB, ELISA, ICC/IF, IHC
• Clone: 3F8
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

GRK2 Antibody (3F8) Summary

• Immunogen: Purified recombinant fragment of human GRK2 expressed in E. Coli.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: GRK2
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • ELISA 1:10000
  • Immunocytochemistry/ Immunofluorescence 1:200 - 1:1000
  • Immunohistochemistry 1:200 - 1:1000
  • Immunohistochemistry-Paraffin 1:200 - 1:1000
  • Western Blot 1:500 - 1:2000
• Theoretical MW: 80 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Ascites
• Preservative: 0.03% Sodium Azide
• Purity: Unpurified

Alternate Names for GRK2 Antibody (3F8)

• ADRBK1
• adrenergic, beta, receptor kinase 1
• BARK
• BARK1
• BARK1beta-ARK-1
• Beta-ARK1
• Beta-ARK-1
• EC 2.7.11
• EC 2.7.11.15
• FLJ16718
• G-protein coupled receptor kinase 2
• GRK2
• GRK2beta-adrenergic receptor kinase 1

Background

Adrenergic Receptor beta Kinase 1 (ADRBK1), also known as GRK2, is a member of the G-protein receptor kinase family. A ubiquitous cytosolic enzyme, GRK-2 specifically phosphorylates the beta-2-adrenergic receptor and appears to mediate agonist-specific desensitization observed under high agonist concentrations. Defects in GRK2 coupling with G-protein receptors may lead to heart failure.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for GRK2 Antibody (NBP2-37611)(1)

Publication using NBP2-37611

• Nakano T, Onoue K, Nakada Y et al. Alteration of b-Adrenoceptor Signaling in Left Ventricle of Acute Phase Takotsubo Syndrome: a Human Study Sci Rep 2018-08-24 [PMID: 30143703] (IF/IHC, Human)

Bioinformatics

• Gene Symbol: GRK2