GPRC5D Antibody (571932) [Unconjugated]

HEK293 human embryonic kidney cell line transfected with either (A) Human GPRC5D or (B) irrelevant protein, and eGFP was stained with Mouse anti-Human GPRC5D Monoclonal Antibody (Catalog # MAB63001) followed by Allophycocyanin-conjugated anti-Mouse IgG Secondary Antibody (F0101B). Quadrant markers were set based on isotype control staining (MAB003, data not shown). Staining was performed using our Staining Membrane-Associated Proteins protocol.

• Reactivity: Hu
• Applications: Flow, CyTOF-ready
• Clone: 571932
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

GPRC5D Antibody (571932) [Unconjugated] Summary

• Immunogen: NS0 mouse myeloma cell line transfected with human GPRC5D
  Met1-Val359
  Accession # Q9NZD1
• Specificity: Detects human GPRC5D in direct ELISAs.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for GPRC5D Antibody (571932) [Unconjugated]

• G protein-coupled receptor, family C, group 5, member D
• GPRC5D
• G-protein coupled receptor family C group 5 member D
• MGC129713
• MGC129714
• orphan G-protein coupled receptor

Background

G-protein coupled receptor family C group 5 member D (GPRC5D) is a retinoic acid-inducible 40 kDa protein with seven transmembrane segments and a short N‑terminal extracellular region. It is expressed in keratinizing epithelial cells, the pancreas, kidney, small intestine, spleen, and testis. Alternate splicing generates two additional isoforms with substitutions or truncations in the cytoplasmic tail. Human GPRC5D shares approximately 80% aa sequence identity with mouse and rat GPRC5D.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Uniprot:
  • Q9NZD1(Human)