GPRC5B Antibody (575926) [Unconjugated]

HEK293 human embryonic kidney cell line transfected with (A) GPRC5B or (B) irrelevant protein, and eGFP were stained with Mouse Anti-Human GPRC5B Monoclonal Antibody (Catalog # MAB10253) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). Quadrant markers were set based Mouse IgG1 Isotype Control Antibody staining (Catalog # MAB003, data not shown). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Hu
• Applications: Flow, CyTOF-ready
• Clone: 575926
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

GPRC5B Antibody (575926) [Unconjugated] Summary

• Immunogen: NS0 mouse myeloma cell line transfected with human GPRC5B
  Accession # Q9NZH0
• Specificity: Detects human GPRC5B.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for GPRC5B Antibody (575926) [Unconjugated]

• G protein-coupled receptor, family C, group 1, member B
• G protein-coupled receptor, family C, group 5, member B
• GPRC5B
• RAIG2
• RAIG-2A-69G12.1
• RAIG2G-protein coupled receptor family C group 5 member B
• retinoic acid responsive gene protein
• Retinoic acid-induced gene 2 protein

Background

G-protein coupled receptor family C group 5-member B (GPRC5B) is a retinoic acid-inducible protein that belongs to the seven-transmembrane domain family of receptors. Human GPRC5B shares approximately 85% aa sequence identity with mouse GPRC5B.

Although GPRC5B is considered an "orphan receptor" and its molecular function is not completely understood, it has been implicated in obesity-associated inflammatory signaling in adipocytes and in diet-induced insulin resistance. Consistent with that, CRISPR-Cas9-mediated knock-down of the GPRC5B gene allowed the identification of signaling pathways linking the GPRC5B receptor expression to beta -cell proliferation and apoptosis.

 

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Uniprot:
  • Q9NZH0()