GPR74/NPFFR2 Antibody

Product Discontinued

GPR74/NPFFR2 Antibody Summary

• Epitope: N-terminal extracellular domain of human
• Specificity: Human NPFFR2. BLAST analysis of the peptide immunogen showed no homology with other human proteins, except MFN2 (45%), MFN1 (45%).
• Predicted Species: Primate (95%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: NPFFR2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1-2 ug/ml

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Gorilla (100%), Gibbon (95%).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.1% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for GPR74/NPFFR2 Antibody

• GPR74
• GPR74G protein-coupled receptor 74
• G-protein coupled receptor 74
• neuropeptide FF 2
• neuropeptide FF receptor 2
• Neuropeptide G-protein coupled receptor
• NPFF2
• NPFF2G-protein coupled receptor HLWAR77
• NPFFR2
• NPGPR
• NPGPRHLWAR77

Background

The Neuropeptide FF 2 Receptor (NPFF2/HLWAR77/NPGPR) is a Neuropeptide Receptor GPCR. This receptor has high affinity for NPAF and NPFF, and is also weakly activated by FMRF-amide (Phe-Met-Arg-Phe-amide) and a variety of related peptides. The receptor is expressed in brain regions associated with opiate activity, consistent with the pain-modulating activity of these peptides, and in adipose tissue, suggesting other physiological and pathophysiological activities for FMRF-amide neuropeptides (Elshourbagy et al, 2000). Two isoforms are produced by alternative splicing. NPFF2 receptor has been reported to be expressed in various tissues, including brain, heart, intestine, placenta, prostate, spleen, adipose, spinal cord, colon, kidney, liver, lung, ovary, testis, pancreas, and thymus. ESTs have been isolated from fetal and placenta libraries.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

FAQs for GPR74/NPFFR2 Antibody (NLS463). (Showing 1 - 1 of 1 FAQ).

1. Is it possible to get more definitive information about the immunogen used to generate the antibiody NLS463? There is a long form and a short form for NPFFR2, the long form has a distinct N-terminus.
   • NLS463 is raised against a 20 amino acid synthetic peptide located between aa 90-140 of the canonical human NPFFR2. The long form is the canonical sequence (Q9Y5X5).

Bioinformatics

• Gene Symbol: NPFFR2
• Entrez:
  • 10886(Human)