GPR37 Antibody (1036313) [Unconjugated]

Western blot shows lysates of human brain (motor cortex) and K562 human chronic myelogenous leukemia cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human GPR37 Monoclonal Antibody (Catalog # MAB44501) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF018). A specific band was detected for GPR37 at approximately 74 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

• Reactivity: Hu
• Applications: WB
• Clone: 1036313
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

GPR37 Antibody (1036313) [Unconjugated] Summary

• Immunogen: Chinese Hamster Ovary cell line CHO-derived human GPR37
  Leu28-Met265
  Accession # O15354
• Specificity: Detects human GPR37 in direct ELISAs.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot 2 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for GPR37 Antibody (1036313) [Unconjugated]

• EDNRBL
• Endothelin B receptor-like protein 1
• ETBR-LP-1
• G protein-coupled receptor 37 (endothelin receptor type B-like)
• GPR37
• hET(B)R-LP
• PAELR
• PAELRETBR-LP-1
• Parkin-associated endothelin receptor-like receptor
• probable G-protein coupled receptor 37

Background

GPR37 (G-protein coupled receptor 37), also called ETBR-LP-1 (endothelin B receptor-like protein 1) or PAELR (Parkin-associated endothelin receptor-like receptor) is a 587 aa, 7-transmembrane receptor for the neuroprotective and glioprotective factor prosaposin  (1, 4).  It is mainly expressed in neuronal cells, particularly in cerebellar Purkinje cells and the hippocampus (2). It is a substrate of the E3 ubiquitin ligase, parkin, which is up-regulated during endoplasmic reticulum stress (3). In a juvenile form of Parkinson's disease, GPR37 accumulates, contributing to stress-induced neuronal cell death (2). The  extracelluar domains (aa 27-265) of human and mouse GPR37 share 68% aa identity.

1. Marazziti, D. et al. (1997) Genomics, 45:68.
2. Donohue, P.J. et al. (1998) Brain Res Mol Brain Res. 54:152.
3. Omura, T. et al. (2006) J Neurochem. 99:1456.
4. Meyer, R.C. et al. (2013) Proc. Natl. Acad. Sci. U. S. A. 110: 9529

 

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for GPR37 Antibody (MAB44501)(1)

Publication using MAB44501

• Chen, C;Wan, M;Peng, X;Zhang, Q;Liu, Y; GPR37-centered ceRNA network contributes to metastatic potential in lung adenocarcinoma: Evidence from high-throughput sequencing Translational oncology 2023-11-15 [PMID: 37979558] (Western Blot, Human)

Bioinformatics

• Uniprot:
  • O15354()