GPR146 Antibody

Images

 
Immunohistochemistry-Paraffin: GPR146 Antibody [NBP1-02659] - Human Breast, Carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Immunohistochemistry-Paraffin: GPR146 Antibody [NBP1-02659] - Staining of human kidney. Formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 14 ug/ml.
Immunohistochemistry-Paraffin: GPR146 Antibody [NBP1-02659] - HYuman Lung, Small Cell Carcinoma. munohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Product Details

Summary
Reactivity Hu, MkSpecies Glossary
Applications ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

GPR146 Antibody Summary

Immunogen
Synthetic 20 amino acid peptide from C-terminal cytoplasmic domain of human GPR146.
Specificity
Human GPR146. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
Predicted Species
Monkey (90%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
GPR146
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry 14 ug/ml
  • Immunohistochemistry-Paraffin 14 ug/ml
Reviewed Applications
Read 1 Review rated 4
using
NBP1-02659 in the following applications:

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Gorilla (95%), Gibbon (90%), Marmoste (85%).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.1% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for GPR146 Antibody

  • G protein-coupled receptor 146
  • G protein-coupled receptor PGR8
  • GPR146
  • PGR8
  • PGR8G-protein coupled receptor PGR8
  • probable G-protein coupled receptor 146

Background

GPR146 is an Orphan-U GPCR with an unknown ligand, but is likely to be a chemokine receptor.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for GPR146 Antibody (NBP1-02659) (0)

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Review for GPR146 Antibody (NBP1-02659) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: IF.

Reviews using NBP1-02659:
Filter by Applications
IF
(1)
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Human
(1)
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Images Ratings Applications Species Date Details
Immunofluorescence GPR146 NBP1-02659
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reviewed by:
Anonymous
IF Human 04/06/2011
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Summary

ApplicationImmunofluorescence
Sample TestedIn vitro cultured colon cancer cells
SpeciesHuman
CommentsParaformaldehyde fixation/TritonX-100 permeabilizationClear membrane staining on colon cancer cells grown as cellular aggregates (dissociated before IF)

Blocking

Blocking DetailsBlocking Buffer: PBS 3% BSA, Blocking Time: 30 minutes, Blocking Temp: 25 degrees Celsius

Primary Anitbody

Dilution RatioIncubation Dilution Buffer: PBS 3%BSA, 0,05%Tween, 0,01%NaN3, Dilution Ratio: 1:50, Incubation Time: 1hr, Incubation Temp: 37C

Secondary Antibody

Secondary DescriptionSecondary Ab: AlexaFLuor555 conjugated anti-Rabbit whole IgG (H+L), Secondary Ab Dilution: 1:1000
Secondary Manufacturer Cat#A21428

Details

Detection NotesDetection Visualization Method: Fluorescence at 580nm, Negative Control: Rabbit serum 1:500
Fixation DetailsSample Preparation: PFA fixed

Comments

CommentsParaformaldehyde fixation/TritonX-100 permeabilizationClear membrane staining on colon cancer cells grown as cellular aggregates (dissociated before IF)

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

ICC/IF Video Protocol

FAQs for GPR146 Antibody (NBP1-02659) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

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Recent Reviews

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4
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1
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Anonymous
04/06/2011
Application: IF
Species: Human

Bioinformatics

Gene Symbol GPR146
Entrez
Uniprot