GPR109B/HM74 Antibody

Product Discontinued

GPR109B/HM74 Antibody Summary

• Immunogen: Synthetic peptide, corresponding to amino acids 300-350 of Human HM74.
• Specificity: HM74 (T327) detects endogenous levels of HM74 protein.
• Clonality: Polyclonal
• Host: Rabbit
• Gene: HCAR3
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Western Blot 1:500-1:1000
• Application Notes: Western blot (WB) analysis of HM74 (T327) pAb in extracts from RAW264.7 cells.
• Theoretical MW: 45 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2)
• Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for GPR109B/HM74 Antibody

• G protein-coupled receptor 109B
• GPR109B
• G-protein coupled receptor 109B
• G-protein coupled receptor HM74
• G-protein coupled receptor HM74B
• GTP-binding protein
• HCA3
• HCAR3
• HM74
• HM74B
• HM74Puma-g
• Niacin receptor 2
• NIACR2
• Nicotinic acid receptor 2
• PUMAG
• putative chemokine receptor

Background

This is a receptor for nicotinic acid. Acts as a low affinity receptor for nicotinic acid and mediate its anti-lipolytic effect through a G(i)-protein-mediated inhibition of adenylyl cyclase. This pharmacological effect requires nicotinic acid doses that are much higher than those provided by a normal diet. Expression in humans is largely restricted to adipose tissue and spleen but there are some reports of it being expressed in lymphocytes and monocytes. In mouse is expressed in lungs, spleen, heart, skeletal muscle and adipose tissue. In rat it is expressed in adipose tissue, lung and spleen. ESTs have been isolated from adipose, blood, liver, liver/spleen, placenta, prostate, and testis libraries.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for GPR109B/HM74 Antibody (NBP1-00875)(2)

Publications using NBP1-00875

• Parodi B, Rossi S, Morando S et al. Fumarates modulate microglia activation through a novel HCAR2 signaling pathway and rescue synaptic dysregulation in inflamed CNS Acta Neuropathol. 2015-04-29 [PMID: 25920452] (ICC/IF, Mouse)
• Li X, Millar JS, Brownell N et al. Modulation of HDL metabolism by the niacin receptor GPR109A in mouse hepatocytes Biochem Pharmacol 2010-11-01 [PMID: 20655299] (WB, Human)

Bioinformatics

• Gene Symbol: HCAR3
• Entrez:
  • 8843(Human)
  • 353250(Rat)