Glutathione S-Transferase mu 1/GSTM1 Antibody (1H4A4)

Images

 
Western Blot: Glutathione S-Transferase mu 1/GSTM1 Antibody (1H4A4) [NBP2-22185] - Western blot analysis using GSTM1 mouse mAb against Cos7 (1), MCF-7 (2), Jurkat (3), Hela (4), HL7702 (5) and HepG2 (6) cell lysate.
Immunohistochemistry-Paraffin: Glutathione S-Transferase mu 1/GSTM1 Antibody (1H4A4) [NBP2-22185] - Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using GSTM1 mouse mAb with DAB staining.
Flow Cytometry: Glutathione S-Transferase mu 1/GSTM1 Antibody (1H4A4) [NBP2-22185] - Flow cytometric analysis of Jurkat cells using GSTM1 mouse mAb (green) and negative control (red).
Western Blot: Glutathione S-Transferase mu 1/GSTM1 Antibody (1H4A4) [NBP2-22185] - Western blot analysis using GSTM1 mAb against human GSTM1 (AA: 23-181) recombinant protein. (Expected MW is 25.7 kDa)
Immunohistochemistry-Paraffin: Glutathione S-Transferase mu 1/GSTM1 Antibody (1H4A4) [NBP2-22185] - Immunohistochemical analysis of paraffin-embedded liver cancer tissues using GSTM1 mouse mAb with DAB staining.
ELISA: Glutathione S-Transferase mu 1/GSTM1 Antibody (1H4A4) [NBP2-22185] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng)

Product Details

Summary
Reactivity Hu, PmSpecies Glossary
Applications WB, ELISA, Flow, IHC, IHC-P
Clone
1H4A4
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

Glutathione S-Transferase mu 1/GSTM1 Antibody (1H4A4) Summary

Immunogen
Purified recombinant fragment of human GSTM1 expressed in E. Coli.
Localization
Cytoplasm
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
GSTM1
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:2000
  • ELISA 1:10000
  • Flow Cytometry 1:200-1:400
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:200-1:1000
Theoretical MW
26 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Ascites
Preservative
0.03% Sodium Azide
Purity
Unpurified

Alternate Names for Glutathione S-Transferase mu 1/GSTM1 Antibody (1H4A4)

  • EC 2.5.1.18
  • glutathione S-alkyltransferase
  • glutathione S-aralkyltransferase
  • glutathione S-aryltransferase
  • glutathione S-transferase M1
  • Glutathione STransferase mu 1
  • Glutathione S-Transferase mu 1
  • GST class-mu 1
  • GST HB subunit 4
  • GST1
  • GSTM1
  • GSTM1-1
  • GSTM1a-1a
  • GSTM1b-1b
  • GTH4
  • GTM1
  • HB subunit 4
  • H-B
  • MGC26563
  • MU
  • MU-1
  • S-(hydroxyalkyl)glutathione lyase

Background

Cytosolic and membrane-bound forms of glutathione S-transferase are encoded by two distinct supergene families. At present, eight distinct classes of the soluble cytoplasmic mammalian glutathione S-transferases have been identified: alpha, kappa, mu, omega, pi, sigma, theta and zeta. This gene encodes a glutathione S-transferase that belongs to the mu class. The mu class of enzymes functions in the detoxification of electrophilic compounds, including carcinogens, therapeutic drugs, environmental toxins and products of oxidative stress, by conjugation with glutathione. The genes encoding the mu class of enzymes are organized in a gene cluster on chromosome 1p13.3 and are known to be highly polymorphic. These genetic variations can change an individual's susceptibility to carcinogens and toxins as well as affect the toxicity and efficacy of certain drugs. Null mutations of this class mu gene have been linked with an increase in a number of cancers, likely due to an increased susceptibility to environmental toxins and carcinogens. Multiple protein isoforms are encoded by transcript variants of this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol GSTM1
Entrez