Human Glutathione Peroxidase 3/GPX3 ELISA Kit (Colorimetric) Summary
Description |
This Human Glutathione Peroxidase 3/GPX3 ELISA Kit (Colorimetric) is to be used for the in vitro quantitative determination of human Glutathione Peroxidase 3/GPX3 in serum, plasma and cell culture supernatant. |
Standard Curve Range |
0.5 - 32 ng/mL |
Sensitivity |
100 pg/mL |
Assay Type |
Sandwich ELISA (quantitative) |
Inter-Assay |
Sample 1: CV = 4.83% (n=8)
Sample 2: CV = 4.77% (n=8)
Sample 3: CV = 4.17% (n=8)
Sample 4: CV = 4.09% (n=8)
Sample 5: CV = 4.66% (n=8)
Sample 6: CV = 1.12% (n=8)
Sample 7: CV = 4.79% (n=8)
Sample 8: CV = 5.04% (n=8) |
Intra-Assay |
Sample 1: CV = 5.04% (n=12)
Sample 2: CV = 4.77% (n=12)
Sample 3: CV = 5.03% (n=12)
Sample 4: CV = 8.46% (n=12)
Sample 5: CV = 8.35% (n=12)
Sample 6: CV = 9.64% (n=12)
Sample 7: CV = 4.21% (n=12)
Sample 8: CV = 4.24% (n=12) |
Spike Recovery |
92% |
Sample Volume |
100 uL |
Kit Type |
ELISA Kit (Colorimetric) |
Gene |
GPX3 |
Applications/Dilutions
Packaging, Storage & Formulations
Storage |
Storage of components varies. See protocol for specific instructions. |
Kit Components
Components
|
- 1 bottle Stop Solution (STOP)
- 1 bottle TMB Substrate Solution (TMB)
- 1 plate coated with human GPX3 Antibody
- 1 vial Detection Antibody (DET)
- 1 vial HRP Labeled Streptavidin (lyophilized) (STREP-HEP)
- 1 vial human GPX3 Standard (lyophilized) (STD)
- 2 bottles ELISA Buffer 10X
- 2 bottles Wash Buffer 10X
- 2 plate sealers (plastic film)
- 2 silica Gel Minibags
|
Alternate Names for Human Glutathione Peroxidase 3/GPX3 ELISA Kit (Colorimetric)
Background
Glutathione Peroxidase 3 (GPX3) belongs to the glutathione peroxidase family. It protects cells and enzymes from oxidative damage by catalyzing the reduction of hydrogen peroxide, lipid peroxides and organic hydroperoxide by glutathione. There is increasing evidence that GPX3 is a novel tumor suppressor and a therapeutic target for insulin resistance and diabetes mellitus.
GPX-3 antibodies are useful tools for research on cellular response to oxidative stress as well as cancer and diabetes studies.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are
guaranteed for 6 months from date of receipt.
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