Human Glutathione Peroxidase 3/GPX3 ELISA Kit (Colorimetric)

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ELISA: Human Glutathione Peroxidase 3/GPX3 ELISA Kit (Colorimetric) - Sample standard curve for Human Glutathione Peroxidase 3/GPX3 ELISA Kit (Colorimetric)

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA
Suitable Sample Type
Serum, plasma, or cell culture supernatant
Standard Curve Range
0.5 - 32 ng/mL
Sensitivity
100 pg/mL

Order Details

Human Glutathione Peroxidase 3/GPX3 ELISA Kit (Colorimetric) Summary

Description
This Human Glutathione Peroxidase 3/GPX3 ELISA Kit (Colorimetric) is to be used for the in vitro quantitative determination of human Glutathione Peroxidase 3/GPX3 in serum, plasma and cell culture supernatant.
Standard Curve Range
0.5 - 32 ng/mL
Sensitivity
100 pg/mL
Assay Type
Sandwich ELISA (quantitative)
Inter-Assay
Sample 1: CV = 4.83% (n=8) Sample 2: CV = 4.77% (n=8) Sample 3: CV = 4.17% (n=8) Sample 4: CV = 4.09% (n=8) Sample 5: CV = 4.66% (n=8) Sample 6: CV = 1.12% (n=8) Sample 7: CV = 4.79% (n=8) Sample 8: CV = 5.04% (n=8)
Intra-Assay
Sample 1: CV = 5.04% (n=12) Sample 2: CV = 4.77% (n=12) Sample 3: CV = 5.03% (n=12) Sample 4: CV = 8.46% (n=12) Sample 5: CV = 8.35% (n=12) Sample 6: CV = 9.64% (n=12) Sample 7: CV = 4.21% (n=12) Sample 8: CV = 4.24% (n=12)
Spike Recovery
92%
Sample Volume
100 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
GPX3

Applications/Dilutions

Dilutions
  • ELISA
  • Sandwich ELISA

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. 1 bottle Stop Solution (STOP)
  2. 1 bottle TMB Substrate Solution (TMB)
  3. 1 plate coated with human GPX3 Antibody
  4. 1 vial Detection Antibody (DET)
  5. 1 vial HRP Labeled Streptavidin (lyophilized) (STREP-HEP)
  6. 1 vial human GPX3 Standard (lyophilized) (STD)
  7. 2 bottles ELISA Buffer 10X
  8. 2 bottles Wash Buffer 10X
  9. 2 plate sealers (plastic film)
  10. 2 silica Gel Minibags

Alternate Names for Human Glutathione Peroxidase 3/GPX3 ELISA Kit (Colorimetric)

  • EC 1.11.1
  • EC 1.11.1.9
  • Extracellular glutathione peroxidase
  • glutathione peroxidase 3 (plasma)
  • Glutathione Peroxidase 3
  • GPX3
  • GPx-3
  • GPXP
  • GPX-P
  • GSHPx-3
  • GSHPx-P
  • Plasma glutathione peroxidase

Background

Glutathione Peroxidase 3 (GPX3) belongs to the glutathione peroxidase family. It protects cells and enzymes from oxidative damage by catalyzing the reduction of hydrogen peroxide, lipid peroxides and organic hydroperoxide by glutathione. There is increasing evidence that GPX3 is a novel tumor suppressor and a therapeutic target for insulin resistance and diabetes mellitus.

GPX-3 antibodies are useful tools for research on cellular response to oxidative stress as well as cancer and diabetes studies.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Bioinformatics

Gene Symbol GPX3