Human Glutathione Peroxidase 3/GPX3 ELISA Kit (Colorimetric)

ELISA: Human Glutathione Peroxidase 3/GPX3 ELISA Kit (Colorimetric) - Sample standard curve for Human Glutathione Peroxidase 3/GPX3 ELISA Kit (Colorimetric)

• Reactivity: Hu
• Applications: ELISA
• Suitable Sample Type: Serum, plasma, or cell culture supernatant
• Standard Curve Range: 0.5 - 32 ng/mL
• Sensitivity: 100 pg/mL

Human Glutathione Peroxidase 3/GPX3 ELISA Kit (Colorimetric) Summary

• Description: This Human Glutathione Peroxidase 3/GPX3 ELISA Kit (Colorimetric) is to be used for the in vitro quantitative determination of human Glutathione Peroxidase 3/GPX3 in serum, plasma and cell culture supernatant.
• Standard Curve Range: 0.5 - 32 ng/mL
• Sensitivity: 100 pg/mL
• Assay Type: Sandwich ELISA (quantitative)
• Inter-Assay: Sample 1: CV = 4.83% (n=8) Sample 2: CV = 4.77% (n=8) Sample 3: CV = 4.17% (n=8) Sample 4: CV = 4.09% (n=8) Sample 5: CV = 4.66% (n=8) Sample 6: CV = 1.12% (n=8) Sample 7: CV = 4.79% (n=8) Sample 8: CV = 5.04% (n=8)
• Intra-Assay: Sample 1: CV = 5.04% (n=12) Sample 2: CV = 4.77% (n=12) Sample 3: CV = 5.03% (n=12) Sample 4: CV = 8.46% (n=12) Sample 5: CV = 8.35% (n=12) Sample 6: CV = 9.64% (n=12) Sample 7: CV = 4.21% (n=12) Sample 8: CV = 4.24% (n=12)
• Spike Recovery: 92%
• Sample Volume: 100 uL
• Kit Type: ELISA Kit (Colorimetric)
• Gene: GPX3

Applications/Dilutions

• Dilutions:
  • ELISA
  • Sandwich ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Human Glutathione Peroxidase 3/GPX3 ELISA Kit (Colorimetric)

• EC 1.11.1
• EC 1.11.1.9
• Extracellular glutathione peroxidase
• glutathione peroxidase 3 (plasma)
• Glutathione Peroxidase 3
• GPX3
• GPx-3
• GPXP
• GPX-P
• GSHPx-3
• GSHPx-P
• Plasma glutathione peroxidase

Background

Glutathione Peroxidase 3 (GPX3) belongs to the glutathione peroxidase family. It protects cells and enzymes from oxidative damage by catalyzing the reduction of hydrogen peroxide, lipid peroxides and organic hydroperoxide by glutathione. There is increasing evidence that GPX3 is a novel tumor suppressor and a therapeutic target for insulin resistance and diabetes mellitus.

GPX-3 antibodies are useful tools for research on cellular response to oxidative stress as well as cancer and diabetes studies.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

FAQs for Glutathione Peroxidase 3/GPX3 ELISA Kit (NBP2-78850). (Showing 1 - 1 of 1 FAQ).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: GPX3