Glucosaminyl (N-acetyl) Transferase 1/GCNT1 Overexpression Lysate


Western Blot: GCNT1 Overexpression Lysate (Adult Normal) [NBL1-11014] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for GCNT1.

Product Details

Reactivity HuSpecies Glossary
Applications WB

Order Details

Glucosaminyl (N-acetyl) Transferase 1/GCNT1 Overexpression Lysate Summary


Glucosaminyl (N-acetyl) Transferase 1/GCNT1 Transient Overexpression Lysate

Expression Host: HEK293T

Plasmid: RC214131

Accession#: NM_001490

Protein Tag: C-MYC/DDK


  • Western Blot
Application Notes
This product is intended for use as a positive control in Western Blot.

You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul).
Theoretical MW
49.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Store at -80C. Avoid freeze-thaw cycles.
RIPA buffer

Lysate Details for Array



HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for Glucosaminyl (N-acetyl) Transferase 1/GCNT1 Overexpression Lysate

  • beta-1,3-galactosyl-O-glycosyl-glycoproteinbeta-1,6-N-acetylglucosaminyltransferase
  • beta-1,6-N-acetylglucosaminyltransferase
  • C2GNT1
  • C2GNTbeta-1,3-galactosyl-O-glycosyl-glycoproteinbeta-1,6-N--acetylglucosaminyltransferase
  • C2GNT-L
  • core 2 beta1,6 N-acetylglucosaminyltransferase-I
  • core 2 beta-1,6-N-acetylglucosaminyltransferase I
  • core 2 branching enzyme
  • Core 2 GNT
  • Core 2-branching enzyme
  • core2-GlcNAc-transferase
  • EC
  • G6NT
  • GCNT1
  • glucosaminyl (N-acetyl) transferase 1, core 2
  • glucosaminyl (N-acetyl) transferase 1, core 2(beta-1,6-N-acetylglucosaminyltransferase)
  • MGC126335
  • MGC126336
  • NACGT2
  • NAGCT2


This gene is a member of the beta-1,6-N-acetylglucosaminyltransferase gene family. It is essential to the formation of Gal beta 1-3(GlcNAc beta 1-6)GalNAc structures and the core 2 O-glycan branch. The gene coding this enzyme was originally mapped to 9q21, but was later localized to 9q13. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq]. Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1, and uses the P2 promoter. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Gene Symbol GCNT1