Glucosaminyl (N-acetyl) Transferase 1/GCNT1 Overexpression Lysate

Images

 
Western Blot: GCNT1 Overexpression Lysate (Adult Normal) [NBL1-11014] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for GCNT1.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

Glucosaminyl (N-acetyl) Transferase 1/GCNT1 Overexpression Lysate Summary

Description

Glucosaminyl (N-acetyl) Transferase 1/GCNT1 Transient Overexpression Lysate


Expression Host: HEK293T

Plasmid: RC214131

Accession#: NM_001490

Protein Tag: C-MYC/DDK
Gene
GCNT1

Applications/Dilutions

Dilutions
  • Western Blot
Application Notes
This product is intended for use as a positive control in Western Blot.

You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul).
Theoretical MW
49.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Array

Type
Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for Glucosaminyl (N-acetyl) Transferase 1/GCNT1 Overexpression Lysate

  • beta-1,3-galactosyl-O-glycosyl-glycoproteinbeta-1,6-N-acetylglucosaminyltransferase
  • beta-1,6-N-acetylglucosaminyltransferase
  • C2GNT1
  • C2GNTbeta-1,3-galactosyl-O-glycosyl-glycoproteinbeta-1,6-N--acetylglucosaminyltransferase
  • C2GNT-L
  • core 2 beta1,6 N-acetylglucosaminyltransferase-I
  • core 2 beta-1,6-N-acetylglucosaminyltransferase I
  • core 2 branching enzyme
  • Core 2 GNT
  • Core 2-branching enzyme
  • core2-GlcNAc-transferase
  • EC 2.4.1.102
  • G6NT
  • GCNT1
  • glucosaminyl (N-acetyl) transferase 1, core 2
  • glucosaminyl (N-acetyl) transferase 1, core 2(beta-1,6-N-acetylglucosaminyltransferase)
  • MGC126335
  • MGC126336
  • NACGT2
  • NAGCT2

Background

This gene is a member of the beta-1,6-N-acetylglucosaminyltransferase gene family. It is essential to the formation of Gal beta 1-3(GlcNAc beta 1-6)GalNAc structures and the core 2 O-glycan branch. The gene coding this enzyme was originally mapped to 9q21, but was later localized to 9q13. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq]. Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1, and uses the P2 promoter. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP2-32822
Species: Hu
Applications: Flow, ICC/IF, IHC, IHC-P, WB
NBP1-88313
Species: Hu
Applications: ICC/IF, IHC, IHC-P, Simple Western, WB
NB120-22711
Species: Hu, Mu
Applications: ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P
NBP1-88781
Species: Hu
Applications: ICC/IF, IHC, IHC-P
NB100-524
Species: Hu, Mu
Applications: Flow-IC, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, WB
BBA16
Species: Hu
Applications: ELISA(Cap), ELISA(Det), ELISA(Sta), Flow, ICC, IHC, IP, WB
NBP2-79843
Species: Hu
Applications: CyTOF-ready, ELISA, Flow, ICC/IF, IHC, IHC-P, PA, WB
7268-CT
Species: Hu
Applications: BA
137-PS
Species: Hu
Applications: BA
6507-IL/CF
Species: Hu
Applications: BA
AF6465
Species: Hu, Rt
Applications: ICC, WB
NBP2-47489
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
MAB64091
Species: Hu
Applications: ICC, WB
NBL1-11014
Species: Hu
Applications: WB

Publications for Glucosaminyl (N-acetyl) Transferase 1/GCNT1 Lysate (NBL1-11014) (0)

There are no publications for Glucosaminyl (N-acetyl) Transferase 1/GCNT1 Lysate (NBL1-11014).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for Glucosaminyl (N-acetyl) Transferase 1/GCNT1 Lysate (NBL1-11014) (0)

There are no reviews for Glucosaminyl (N-acetyl) Transferase 1/GCNT1 Lysate (NBL1-11014). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for Glucosaminyl (N-acetyl) Transferase 1/GCNT1 Lysate (NBL1-11014) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional Glucosaminyl (N-acetyl) Transferase 1/GCNT1 Products

Bioinformatics Tool for Glucosaminyl (N-acetyl) Transferase 1/GCNT1 Lysate (NBL1-11014)

Discover related pathways, diseases and genes to Glucosaminyl (N-acetyl) Transferase 1/GCNT1 Lysate (NBL1-11014). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for Glucosaminyl (N-acetyl) Transferase 1/GCNT1 Lysate (NBL1-11014)

Discover more about diseases related to Glucosaminyl (N-acetyl) Transferase 1/GCNT1 Lysate (NBL1-11014).

Research Areas for Glucosaminyl (N-acetyl) Transferase 1/GCNT1 Lysate (NBL1-11014)

Find related products by research area.

Blogs on Glucosaminyl (N-acetyl) Transferase 1/GCNT1

There are no specific blogs for Glucosaminyl (N-acetyl) Transferase 1/GCNT1, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Glucosaminyl (N-acetyl) Transferase 1/GCNT1 Overexpression Lysate and receive a gift card or discount.

Bioinformatics

Gene Symbol GCNT1