Glucosaminyl (N-acetyl) Transferase 1/GCNT1 Antibody [Alexa Fluor™ Plus 488]

• Reactivity: Hu
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Alexa Fluor Plus 488

Glucosaminyl (N-acetyl) Transferase 1/GCNT1 Antibody [Alexa Fluor™ Plus 488] Summary

• Specificity: Detects human Glucosaminyl (N-acetyl) Transferase 1/GCNT1 in direct ELISAs and Western blots.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Glucosaminyl (N-acetyl) Transferase 1/GCNT1 Antibody [Alexa Fluor™ Plus 488]

• beta-1,3-galactosyl-O-glycosyl-glycoproteinbeta-1,6-N-acetylglucosaminyltransferase
• beta-1,6-N-acetylglucosaminyltransferase
• C2GNT1
• C2GNTbeta-1,3-galactosyl-O-glycosyl-glycoproteinbeta-1,6-N--acetylglucosaminyltransferase
• C2GNT-L
• core 2 beta1,6 N-acetylglucosaminyltransferase-I
• core 2 beta-1,6-N-acetylglucosaminyltransferase I
• core 2 branching enzyme
• Core 2 GNT
• Core 2-branching enzyme
• core2-GlcNAc-transferase
• EC 2.4.1.102
• G6NT
• GCNT1
• glucosaminyl (N-acetyl) transferase 1, core 2
• glucosaminyl (N-acetyl) transferase 1, core 2(beta-1,6-N-acetylglucosaminyltransferase)
• MGC126335
• MGC126336
• NACGT2
• NAGCT2

Background

Mucin-type O-glycans are initiated with an O-GalNAc attachment to a serine or threonine in a polypeptide. The O-GalNAc residues are subsequently extended by various glycosyltransferases resulting in different types of O-glycans. Most O-glycans contain the core 1 structure, Gal beta 1-3GalNAc. Glucosaminyl (N-acetyl) Transferase 1 (GCNT1) converts the core 1 O-glycan to core 2 O-glycan, Gal beta 1-3(GlcNAc beta 1-6)GalNAc, via the addition of a GlcNAc residue (1, 2). Various ligand carbohydrates can be formed from core 2 branched oligosaccharides. For example, sialyl Le^x in mucin‑type glycoproteins of blood cells can be formed from core 2 branched oligosaccharides (3, 4). The expression of GCNT1 was found to be associated with the progression of various types of cancer (5, 6, 7). The enzymatic activity of the recombinant GCNT1 is measured using a phosphatase-coupled method (8).

1. Bierhuizen, M.F. (1993) Genes & Development 7:468.
2. Yeh, J.C. et al. (1999) J. Biol. Chem. 274:3215.
3. Hemmerich, S. et al. (1995) J. Biol. Chem. 270:12035.
4. Wilkins, P.P. et al. (1996) J. Biol. Chem. 270:18732.
5. Shimodaira, K. et al. (1997) Cancer Res. 57: 5201.
6. Hatakyama, S. et al. (2010) Int. J. Cancer 127:1052.
7. St Hill, C.A. et al. (2009) BMC Cancer 9:79.
8. Wu, Z.L. et al. (2011) Glycobiology 21:727.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.