Mouse GIP ELISA Kit (Colorimetric)

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ELISA: Mouse GIP ELISA Kit (Colorimetric) [NBP2-82162] - Samples were spiked with high concentrations of Mouse GIP and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of ...read more
ELISA: Mouse GIP ELISA Kit (Colorimetric) [NBP2-82162] - Standard Curve Reference

Product Details

Summary
Reactivity MuSpecies Glossary
Applications ELISA
Suitable Sample Type
Serum, Plasma, Cell supernatant
Standard Curve Range
78.13~5000pg/mL
Sensitivity
46.88 pg/mL

Order Details

Mouse GIP ELISA Kit (Colorimetric) Summary

Specificity
This kit recognizes Mouse GIP in samples. No Significant cross-reactivity or interference between Mouse GIP and analogues was observed.
Standard Curve Range
78.13~5000pg/mL
Sensitivity
46.88 pg/mL
Assay Type
Sandwich-ELISA
Inter-Assay
CV% < 5%
Intra-Assay
CV% < 5%
Spike Recovery
average = 94% for serum; average = 97% for EDTA plasma; average = 95% for cell culture media
Sample Volume
100 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
GIP

Applications/Dilutions

Dilutions
  • ELISA

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Micro ELISA Plate, dismountable (8 wells x 12 strips); Reference Standard (2 vials); Concentrated (100X) Biotinylated Detection Ab (120 uL); Concentrated (100X) HRP Conjugate (120 uL); Reference Standard & Sample Diluent (20 mL); Biotinylated Detection Ab

Alternate Names for Mouse GIP ELISA Kit (Colorimetric)

  • gastric inhibitory polypeptide
  • GIP
  • Glucose-dependent Insulinotropic Polypeptide
  • Incretin

Background

The GIP gene encodes an incretin hormone and belongs to the glucagon superfamily. The encoded protein is important in maintaining glucose homeostasis as it is a potent stimulator of insulin secretion from pancreatic beta-cells following food ingestion and nu

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Product General Protocols

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FAQs for GIP ELISA Kit (NBP2-82162). (Showing 1 - 1 of 1 FAQ).

  1. What is the difference between the Quantikine and Duoset ELISA kits?
    • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

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Blogs on GIP.

Inhibiting incretin GIP hormone activity in mouse and monkey models to combat obesity
By Jamshed Arslan, Pharm. D., PhD. We live in a world where 39% of adults are overweight. Our meals trigger the secretion of various gut-derived metabolic hormones called incretins. Fats and carbohydrates in our die...  Read full blog post.

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Bioinformatics

Gene Symbol GIP