GIP Antibody (911205) [Unconjugated]

GIP was detected in immersion fixed paraffin-embedded sections of human stomach using Mouse Anti-Human GIP Monoclonal Antibody (Catalog # MAB8864) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to gastric glands. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

• Reactivity: Hu
• Applications: IHC
• Clone: 911205
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

GIP Antibody (911205) [Unconjugated] Summary

• Immunogen: GIP Peptide
  Tyr1-Asn39
  Accession # P09681
• Specificity: Detects human GIP in direct ELISAs.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: GIP
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 8-25 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for GIP Antibody (911205) [Unconjugated]

• gastric inhibitory polypeptide
• GIP
• Glucose-dependent Insulinotropic Polypeptide
• Incretin

Background

Gastric inhibitory polypeptide (GIP), also known as the glucose-dependent insulinotropic peptide is a member of the secretin family of hormones. GIP, together with glucagon-like peptide-1 (GLP-1), belongs to the group of metabolic hormones called incretins that stimulate a decrease in blood glucose levels. GIP is derived from a 153-amino acid pro-protein encoded by the GIP gene and circulates as a biologically active 42-amino acid peptide.  Engagement of Gastric inhibitory polypeptide receptors (GIPR) by GIP on pancreatic beta cells activates adenylate cyclase to regulate insulin compensation in the presence of high circulating glucose.

GIP (1-39) is an endogenous truncated form of GIP that has been shown to be more potent in stimulating insulin secretion from rat pancreatic islets.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for GIP Antibody (MAB8864)(1)

Publication using MAB8864

• T Masaki, Y Kodera, M Terasaki, K Fujimoto, T Hirano, M Shichiri GIP_HUMAN[22-51] is a new proatherogenic peptide identified by native plasma peptidomics Scientific Reports, 2021;11(1):14470. 2021 [PMID: 34262109] (IHC, Human)

Bioinformatics

• Gene Symbol: GIP
• Uniprot:
  • P09681()