GFR alpha-like Antibody (1045708) [Unconjugated]

GFR alpha ‑like was detected in immersion fixed HEK293 human embryonic kidney cell line transfected (positive staining) and HEK293 human embryonic kidney cell line (non-transfected, negative staining) using Rat Anti-Mouse GFR alpha ‑like Monoclonal Antibody (Catalog # MAB5728) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; NL013) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells.

• Reactivity: Mu
• Applications: ICC/IF
• Clone: 1045708
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated

GFR alpha-like Antibody (1045708) [Unconjugated] Summary

• Immunogen: Chinese Hamster Ovary cell line CHO-derived mouse GFR alpha ‑like
  Gln20-Gly349
  Accession # Q6SJE0-1
• Specificity: Detects mouse GFR alpha ‑like in direct ELISAs.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Rat
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 8-25 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for GFR alpha-like Antibody (1045708) [Unconjugated]

• bA360D14.1
• C6orf144
• GDNF family receptor alpha-like
• GFR alpha like
• GFR alpha-like
• GFRAL
• GRAL
• IVFI9356
• UNQ9356

Background

GFR alpha-like (GDNF receptor-alpha-like) is a 25 kDa dimer and distant member of the GDNFR family of proteins (1). It is expressed on both fetal and adult hindbrain neurons of the CNS (2), and would appear to function as an anti-apoptotic molecule during neuronal stress. GFR alpha-like is a funcation receptor of GDF15 and implicated in diet-based obesity and insulin resistance through c-Ret signaling (2 - 4). Mature mouse GFR alpha-like is a 374 amino acid (aa) type I transmembrane protein. It contains a 330 aa extracellular region that shows a Gas1 domain (aa 220‑316), and a 23 aa cytoplasmic tail. Over aa 19‑353, mouse GFR alpha-like shares 72% and 71% aa identity with human and rat GFR alpha–like, respectively.

1. Li, Z. et al. (2005) J. Neurochem. 95:361.
2. Yang, L. et al. (2017) Nature Medicine 23:1158.
3. Mullican, S. et al. (2017) Nature medicine 23:1150.

4. Emmerson, P. et al. (2017) Nature Medicine 23:1215.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

Uniprot