GCAP2 Antibody (A1) [CoraFluor™ 1]

• Reactivity: Bv
• Applications: WB, ELISA, IHC
• Clone: A1
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: CoraFluor 1

GCAP2 Antibody (A1) [CoraFluor™ 1] Summary

• Description: CoraFluor(TM) 1 is a high performance terbium-based TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or TRF (Time-Resolved Fluorescence) donor for high throughput assay development. CoraFluor(IM) 1 absorbs UV light at approximately 340 nm, and emits at approximately 490 nm, 545 nm, 585 nm and 620 nm. It is compatible with common acceptor dyes that absorb at the emission wavelengths of CoraFluor(TM) 1. CoraFluor(TM) 1 can be used for the development of robust and scalable TR-FRET binding assays such as target engagement, ternary complex, protein-protein interaction and protein quantification assays.
• Immunogen: Bacterial expressed full length GCAP-2.
• Localization: Cell Membrane
• Specificity: This is specific for GCAP 2 and does not react with other isotypes.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: GUCA1B
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark. Do not freeze.
• Buffer: PBS
• Preservative: No Preservative
• Purity: Immunogen affinity purified

Notes

CoraFluor (TM) is a trademark of Bio-Techne Corp. Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254

Alternate Names for GCAP2 Antibody (A1) [CoraFluor™ 1]

• DKFZp686E1183
• GCAP 2
• GCAP2guanylate cyclase-activating protein, photoreceptor 2
• guanylate cyclase activator 1B (retina)
• Guanylate cyclase activator 1B
• guanylyl cyclase-activating protein 2
• GUCA2
• RP48

Background

Guanylate cyclase-activating proteins (GCAPs) are calcium binding proteins that belong to the calmodulin superfamily. GCAPs without calcium are responsible for activation of photoreceptor guanylate cyclase during light adaptation. Studies have shown that the addition or subtraction of Ca2+ results in major conformational changes and the activation/deactivation of GCAP 1 and GCAP 2. It has been demonstrated that both GCAP 1 and 2 act on guanylate cyclase similarly and have approximately 50% homology between the two forms. GCAP-1 is predominantly localized in the photoreceptor outer segments, while GCAP 2 is found in retina samples.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: GUCA1B