Gasdermin D Antibody

Western Blot: GSDMDC1 Antibody [NBP2-80427] - Analysis of Gasdermin D (N terminal) on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: SiHa cell lysateLane 2: human kidney tissue lysate

Immunocytochemistry/Immunofluorescence: GSDMDC1 Antibody [NBP2-80427] - Staining of Gasdermin D in SiHa cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ER1901-37, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).

Immunohistochemistry-Paraffin: GSDMDC1 Antibody [NBP2-80427] - Analysis in Mouse colon tissue using GSDMDC1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with

Flow Cytometry: GSDMDC1 Antibody [NBP2-80427] - Analysis of GSDMDC1 was done on SiHa cells. The cells were fixed, permeabilized and stained with the primary antibody (1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes. Unlabeled sample was used as a control (cells without incubation with primary antibody; black).

Immunohistochemistry-Paraffin: GSDMDC1 Antibody [NBP2-80427] - Analysis in rat stomach tissue using GSDMDC1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with

Immunohistochemistry-Paraffin: GSDMDC1 Antibody [NBP2-80427] - Analysis in human tonsil tissue using GSDMDC1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed wit

Immunohistochemistry-Paraffin: GSDMDC1 Antibody [NBP2-80427] - Analysis in human prostate carcinoma tissue using GSDMDC1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature

Immunohistochemistry-Paraffin: GSDMDC1 Antibody [NBP2-80427] - Analysis in human esophagus tissue using GSDMDC1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed

Western blot analysis of Gasdermin D (N terminal) on THP-1 cell lysates with Gasdermin D Antibody (NBP2-80427) at 1/1,000 dilution.Lysates/proteins at 20 ug/Lane.Predicted band size: 53 kDaObserved band size: 53/35 kDaExposure time: 25 seconds; ECL: K1801;4-20% SDS-PAGE gel.Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.

• Reactivity: Hu, Mu, Rt
• Applications: WB, Flow, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

Gasdermin D Antibody Summary

• Description: Novus Biologicals Rabbit Gasdermin D Antibody (NBP2-80427) is a polyclonal antibody validated for use in IHC, WB, Flow and ICC/IF. Anti-Gasdermin D Antibody: Cited in 6 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein within human Gasdermin D aa 100-300. (SwissProt: P57764 Human; SwissProt: Q9D8T2 Mouse; SwissProt: Q9D8T2 Mouse)
• Localization: Cytosol. Secreted. Plasma membrane.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: GSDMD
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:100
  • Immunocytochemistry/ Immunofluorescence 1:50-1:100
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:500-1:2000
• Theoretical MW: 53 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH7.4), 0.2% BSA, 50% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Gasdermin D Antibody

• DF5L
• DFNA5L
• gasdermin domain containing 1
• Gasdermin domain-containing protein 1
• gasdermin-D
• GSDMDC1

Background

Gasdermin D is a member of the gasdermin family. Members of this family appear to play a role in regulation of epithelial proliferation. Gasdermin D has been suggested to act as a tumor suppressor. Alternatively spliced transcript variants have been described. [provided by RefSeq, Oct 2009]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Gasdermin D Antibody (NBP2-80427)(6)

Publications using NBP2-80427

• Zhou C, Guo Q, Lin J et Al. Single-Cell Atlas of Human Ovaries Reveals The Role Of The Pyroptotic Macrophage in Ovarian Aging Adv Sci (Weinh) 2023-11-30 [PMID: 38036420]
• J Yang, L Miao, Y Xue, X Wang Yiyi Fuzi Baijiang Powder Alleviates Dextran Sulfate Sodium-Induced Ulcerative Colitis in Rats via Inhibiting the TLR4/NF-kappaB/NLRP3 Inflammasome Signaling Pathway to Repair the Intestinal Epithelial Barrier, and Modulating Intestinal Microbiota Oxidative Medicine and Cellular Longevity, 2023-01-14;2023(0):3071610. 2023-01-14 [PMID: 36691639] (Western Blot, Human)
• Li M, Lu H, Wang X Et al. Pyruvate kinase M2 (PKM2) interacts with activating transcription factor 2 (ATF2) to bridge glycolysis and pyroptosis in microglia Molecular immunology 2021-11-17 [PMID: 34798593] (IHC-P, WB, Mouse)
• Song P, Yi Z, Fu Y Et al. Reversing Postcardiopulmonary Bypass Associated Cognitive Dysfunction Using k-Opioid Receptor Agonists to Regulate Microglial Polarization via the NLRP3/Caspase-1 Pathway Journal of healthcare engineering 2021-10-01 [PMID: 34630980] (Rat)
• Shen J, Fan Z, Sun G, Qi G Sacubitril/valsartan (LCZ696) reduces myocardial injury following myocardial infarction by inhibiting NLRP3 induced pyroptosis via the TAK1/JNK signaling pathway Molecular medicine reports 2021-09-01 [PMID: 34296299] (IHC-P)
• Liu F, Liu T, Sun M et al. Maxing Shigan Decoction Mitigates Mycoplasma pneumonia-Induced Pyroptosis in A549 Cells via the NLRP3 Inflammasome Infection and drug resistance 2021-03-03 [PMID: 33688221] (WB, Human)

Bioinformatics

• Gene Symbol: GSDMD