![Western Blot: GSDMDC1 Antibody (8K8C6) [NBP3-33450] - Western blot analysis of lysates from THP-1 cells using GSDMD (Full Length+C terminal) Rabbit mAb at 1:2000 dilution. THP-1 cells were treated by LPS and Nigericin.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.WB samples for antibody validation are kindly provided by Dr. Feng Shao, NIBS](http://images.novusbio.com/fullsize/nbp3-33450_rabbit-gsdmdc1-mab-8k8c6-522025125471.jpg "Western Blot: GSDMDC1 Antibody (8K8C6) [NBP3-33450] - Western blot analysis of lysates from THP-1 cells using GSDMD (Full Length+C terminal) Rabbit mAb at 1:2000 dilution. THP-1 cells were treated by LPS and Nigericin.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.WB samples for antibody validation are kindly provided by Dr. Feng Shao, NIBS")
| Reactivity | HuSpecies Glossary |
| Applications | WB, ELISA |
| Clone | 8K8C6 |
| Clonality | Monoclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Description | Novus Biologicals Rabbit Gasdermin D Antibody (8K8C6) (NBP3-33450) is a monoclonal antibody validated for use in WB and ELISA. All Novus Biologicals antibodies are covered by our 100% guarantee. |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 276-484 of human Gasdermin D) Sequence: GVPAEGAFTEDFQGLRAEVETISKELELLDRELCQLLLEGLEGVLRDQLALRALEEALEQGQSLGPVEPLDGPAGAVLECLVLSSGMLVPELAIPVVYLLGALTMLSETQHKLLAEALESQTLLGPLELVGSLLEQSAPWQERSTMSLPPGLLGNSWGEGAPAWVLLDECGLELGEDTPHVCWEPQAQGRMCALYASLALLSGLSQEPH |
| Isotype | IgG |
| Clonality | Monoclonal |
| Host | Rabbit |
| Gene | GSDMD |
| Purity | Affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7.3), 50% glycerol, 0.05% BSA |
| Preservative | 0.09% Sodium Azide |
| Purity | Affinity purified |
![Myoglobin Antibody [Unconjugated]](/sites/all/modules/enterprise-tech/et_datasheets/images/novus_guarantee.png "Myoglobin Antibody [Unconjugated]")
Secondary Antibodies |
Isotype Controls |
Research Areas for Gasdermin D Antibody (NBP3-33450)Find related products by research area.
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| Gene Symbol | GSDMD |
![Western Blot: GSDMDC1 Antibody (8K8C6) [NBP3-33450] - Western blot analysis of recombinant GSDMD Protein using GSDMD (Full Length+C terminal) Rabbit mAb at 1:2000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 10 ng per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 10s.WB samples for antibody validation are kindly provided by Dr. Feng Shao, NIBS](http://images.novusbio.com/fullsize/nbp3-33450_rabbit-gsdmdc1-mab-8k8c6-5220251141144.jpg "Western Blot: GSDMDC1 Antibody (8K8C6) [NBP3-33450] - Western blot analysis of recombinant GSDMD Protein using GSDMD (Full Length+C terminal) Rabbit mAb at 1:2000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 10 ng per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 10s.WB samples for antibody validation are kindly provided by Dr. Feng Shao, NIBS")
![Western Blot FoxJ3 Antibody [Unconjugated]](https://images.novusbio.com/images2/FoxJ3_AF5786_Western_Blot_5845.jpg)
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")