gamma Tubulin Antibody [Cy3]

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Product Details

Summary
Reactivity Hu, Ca, ChSpecies Glossary
Applications ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Cy3

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gamma Tubulin Antibody [Cy3] Summary

Immunogen
Synthetic peptide: EEFATEGTDRKDVFFY-K, corresponding to amino acids 38-53 of Human gamma Tubulin.
Localization
Centrosomal
Marker
Centrosome Marker
Specificity
Does not react with alpha, beta, omega and eta Tubulin. The IF testing of this product is performed using chicken fibroblasts. We have not performed IF testing using human cells. This antibody is tested in western blotting using the following cell lysates ; U87, HeLa, Jurkat, C2C12, Rat2, MDCK, and A431.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
TUBG1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/Immunofluorescence 1:10-1:2000
Application Notes
IF: Use at a dilution of 1/200. This concentration was determined by staining of methanol/acetonefixed chicken fibroblast cells.Not tested in other applications.Optimal dilutions/concentrations should be determined by the end user.
Theoretical MW
51 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

The antigen sequence is identical in mouse and rat Gamma-tubulin and highly conserved among species (Drosophila, Aspergillus, and yeast Gamma-tubulin).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
10 mM PBS (pH 7.4) and 1.0% BSA
Preservative
0.05% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for gamma Tubulin Antibody [Cy3]

  • gamma-1-tubulin
  • Gamma-tubulin complex component 1
  • GCP-1
  • TUBG
  • TUBGCP1
  • tubulin gamma-1 chain
  • tubulin, gamma 1
  • tubulin, gamma polypeptide

Background

Gamma tubulin, a member of the tubulin superfamily, is a ubiquitous and highly conserved protein within the microtubule organizing centre (MTOC). Gamma tubulin is not a component of microtubules, rather it functions as the microtubule nucleator at the MOTC, is responsible for binding microtubule minus ends and mediating the link between microtubles and the centrosome. By binding to the beta tubulin subunit of the tubulin molecule, it establishes the polarity of a microtubule leaving the alpha tubulin subunit exposed at the positive end. The abundance of Gamma tubulin is less than 1% of the level of either alpha or beta tubulin. It shares approximately 28-32% identity with alpha tubulin from various organisms and 32-36% identity with beta tubulins. The detection, localization and characterization of proteins involved in microtubule function is fundamental to the understanding of mitosis, meiosis and the microtubule cytoskeleton.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol TUBG1