Recombinant Human gamma-Synuclein Active, Monomer Protein

In vitro assay: Recombinant Human gamma-Synuclein Active, Monomer Protein [NBP3-14778] - Thioflavin T is a fluorescent dye that binds to beta sheet-rich structures, such as those in gamma synuclein fibrils. Increased fluorescence (correlated to gamma synuclein fibrillization) is seen when human gamma synuclein monomer (NBP3-14778) is combined with human gamma synuclein PFFs .

SDS-Page: Recombinant Human gamma-Synuclein Active, Monomer Protein [NBP3-14778] - Coomassie gel stain of Human gamma-Synuclein Monomer (NBP3-14778). Load: 2 ug.

• Reactivity: Hu
• Applications: WB, Func, PAGE

Recombinant Human gamma-Synuclein Active, Monomer Protein Summary

• Description: A full length recombinant protein of Human gamma-Synuclein
  
  

  Source: E. coli

  Uniprot ID: O76070

• Localization: Cytoskeleton, Centrosome, Spindle, Perinuclear Region
• Preparation Method: Ion-exchange Purified
• Protein/Peptide Type: Recombinant Protein
• Gene: SNCG
• Purity: >95%, by SDS-PAGE

Applications/Dilutions

• Dilutions:
  • In vitro assay
  • In vivo assay
  • SDS-Page
  • Western Blot

Packaging, Storage & Formulations

• Storage: Store at -80C. Avoid freeze-thaw cycles.
• Buffer: PBS pH 7.4
• Preservative: No Preservative
• Purity: >95%, by SDS-PAGE

Notes

Please note that the 200ug and 500ug sizes are sent in 2x100ug and 5x100ug vials, respectively

Alternate Names for Recombinant Human gamma-Synuclein Active, Monomer Protein

• BCSG1
• BCSG1PERSYN
• Breast cancer-specific gene 1 protein
• gamma-Synuclein
• persyn
• PRSN
• SNCG
• SRPRSN
• Synoretin
• synuclein, gamma (breast cancer-specific protein 1)
• Synuclein-gamma

Background

Plays a role in neurofilament network integrity. May be involved in modulating axonal architecture during development and in the adult. In vitro, increases the susceptibility of neurofilament-H to calcium-dependent proteases. May also function in modulating the keratin network in skin. Activates the MAPK and Elk-1 signal transduction pathway. May be a centrosome-associated protein. Cytoplasm; perinuclear region. Centrosome. Spindle. Associated with centrosomes in several interphase cells. In mitotic cells, localized to the poles of the spindle. Highly expressed in brain, particularly in the substantia nigra. Also expressed in the corpus callosum, heart, skeletal muscle, ovary, testis, colon and spleen. Weak expression in pancreas, kidney and lung. PTM: Phosphorylated. Phosphorylation by GRK5 appears to occur on residues distinct from the residue phosphorylated by other kinases. Brain iron accumulation type 1 (NBIA1, also called Hallervorden-Spatz syndrome), a rare neuroaxonal dystrophy, is histologically characterized by axonal spheroids, iron deposition, Lewy body (LB)-like intraneuronal inclusions, glial inclusions and neurofibrillary tangles. SNCG is found in spheroids but not in inclusions. Belongs to the synuclein family.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: SNCG