GALNT6 Antibody (4C10) - Azide and BSA Free

Western Blot: GALNT6 Antibody (4C10) [H00011226-M01] - GALNT6 monoclonal antibody (M01), clone 4C10 Analysis of GALNT6 expression in A-431.

Western Blot: GALNT6 Antibody (4C10) [H00011226-M01] - Analysis of GALNT6 over-expressed 293 cell line, cotransfected with GALNT6 Validated Chimera RNAi ( Cat # H00011226-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with GALNT6 monoclonal antibody (M01), clone 4C10 (Cat # H00011226-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.

Western Blot: GALNT6 Antibody (4C10) [H00011226-M01] - Analysis of GALNT6 expression in transfected 293T cell line by GALNT6 monoclonal antibody (M01), clone 4C10.Lane 1: GALNT6 transfected lysate(71 KDa).Lane 2: Non-transfected lysate.

Sandwich ELISA: GALNT6 Antibody (4C10) [H00011226-M01] - Detection limit for recombinant GST tagged GALNT6 is approximately 0.1ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA, Mycoplasma
• Clone: 4C10
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

GALNT6 Antibody (4C10) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse GALNT6 Antibody (4C10) - Azide and BSA Free (H00011226-M01) is a monoclonal antibody validated for use in WB and ELISA. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: GALNT6 (NP_009141, 523 a.a. ~ 622 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. IMYSCHGLGGNQYFEYTTQRDLRHNIAKQLCLHVSKGALGLGSCHFTGKNSQVPKDEEWELAQDQLIRNSGSGTCLTSQDKKPAMAPCNPSDPHQLWLFV
• Specificity: GALNT6 - UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase 6 (GalNAc-T6)
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: GALNT6
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Knockdown Validated
  • Sandwich ELISA
  • Western Blot 1:500
• Application Notes: Antibody reactive against cell lysate, transfected lysate and recombinant protein for western blot. It has also been used for RNAi vaidation and ELISA.

Reactivity Notes

Human. Other species not tested.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for GALNT6 Antibody (4C10) - Azide and BSA Free

• EC 2.4.1.41
• GalNAc transferase 6
• GalNAcT6
• GALNAC-T6
• Polypeptide GalNAc transferase 6
• polypeptide N-acetylgalactosaminyltransferase 6
• pp-GaNTase 6
• Protein-UDP acetylgalactosaminyltransferase 6
• UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase 6
• UDP-N-acetyl-alpha-D-galactosamine: polypeptideN-acetylgalactosaminyltransferase 6
• UDP-N-acetyl-alpha-D-galactosamine:polypeptideN-acetylgalactosaminyltransferase 6 (GalNAc-T6)

Background

This gene encodes a member of the UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase (GalNAc-T) family of enzymes. GalNAc-Ts initiate mucin-type O-linked glycosylation in the Golgi apparatus by catalyzing the transfer of GalNAc to serine and threonine residues on target proteins. They are characterized by an N-terminal transmembrane domain, a stem region, a lumenal catalytic domain containing a GT1 motif and Gal/GalNAc transferase motif, and a C-terminal ricin/lectin-like domain. GalNAc-Ts have different, but overlapping, substrate specificities and patterns of expression. The encoded protein is capable of glycosylating fibronectin peptide in vitro and is expressed in a fibroblast cell line, indicating that it may be involved in the synthesis of oncofetal fibronectin.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: GALNT6
• Entrez:
  • 11226(Human)
• OMIM:
  • 605148(Human)
• Uniprot:
  • NP_009141(Human)