Recombinant Human Galectin-8 His Protein

SDS-Page: Recombinant Human Galectin-8 Protein [NBP1-48335] - 15 % SDS-PAGE (3ug)

Product Discontinued

Recombinant Human Galectin-8 His Protein Summary

• Description: A bioactive recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-317 of Human Galectin-8
  
  

  Source: E.coli

  Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MMLSLNNLQN IIYNPVIPFV GTIPDQLDPG TLIVIRGHVP SDADRFQVDL QNGSSMKPRA DVAFHFNPRF KRAGCIVCNT LINEKWGREE ITYDTPFKRE KSFEIVIMVL KDKFQVAVNG KHTLLYGHRI GPEKIDTLGI YGKVNIHSIG FSFSSDLQST QASSLELTEI SRENVPKSGT PQLRLPFAAR LNTPMGPGRT VVVKGEVNAN AKSFNVDLLA GKSKDIALHL NPRLNIKAFV RNSFLQESWG EEERNITSFP FSPGMYFEMI IYCDVREFKV AVNGVHSLEY KHRFKELSSI DTLEINGDIH LLEVRSW

• Details of Functionality: The ED50 for this effect is 5 - 10 ug/ml. Measured by its ability to agglutinate human red blood cells.
• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: LGALS8
• Purity: >90%, by SDS-PAGE

Applications/Dilutions

• Dilutions:
  • Bioactivity
  • SDS-Page
• Theoretical MW: 37.9 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 20 mM Tris-HCl buffer (pH8.0), 0.1 M Nacl, 1 mM DTT, 10% glycerol
• Preservative: No Preservative
• Concentration: 0.5 mg/ml
• Purity: >90%, by SDS-PAGE

Alternate Names for Recombinant Human Galectin-8 His Protein

• GAL8
• Gal-8
• galectin 8
• Galectin8
• Galectin-8
• galectin-8g
• lectin, galactoside-binding, soluble, 8
• LGALS8
• PCTA1
• PCTA-1Po66 carbohydrate-binding protein
• Po66 carbohydrate binding protein
• Po66-CBP
• Prostate carcinoma tumor antigen 1

Background

LGALS8, also known as prostate-specific membrane antigen (PCTA1), is an additional prostate-specific antigen that is overexpressed only in malignant tumors and therefore is a more specific identifier of malignancies. It is a member of the galectin gene family which mediates both cell-cell and cell matrix interactions in a manner similar to the selectin subgroup of C-type lectins. Recombinant human LGALS8 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 1 year from date of receipt.

Publications for Galectin-8 Recombinant Protein (NBP1-48335)(1)

Publication using NBP1-48335

• Zhang L, Yu H, Bai Y Et al. A Neoglycoprotein-Immobilized Fluorescent Magnetic Bead Suspension Multiplex Array for Galectin-Binding Studies Molecules (Basel, Switzerland) 2021-10-14 [PMID: 34684775] (MI)

Bioinformatics

• Gene Symbol: LGALS8