FXR1 Antibody

Western Blot: FXR1 Antibody [NB100-41388] - 1ug/ml staining of NIH-3T3 cell lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

Immunocytochemistry/Immunofluorescence: FXR1 Antibody [NB100-41388] - Paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary antibody at 10 ug/mL, 1 hr incubation followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).

Immunocytochemistry/Immunofluorescence: FXR1 Antibody [NB100-41388] - Paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary antibody at 10 ug/mL, 1 hr incubation, followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).

• Reactivity: Hu, Mu
• Applications: WB, ELISA, Flow, ICC/IF
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

FXR1 Antibody Summary

• Immunogen: Peptide with sequence C-RIEGDNENKLPRED corresponding to internal region according to NP_001013456.1, NP_001013457.1.
• Specificity: This antibody is expected to recognise all reported isoforms (NP_005078.2, NP_001013456.1 and NP_001013457.1).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: FXR1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 10 ug/mL
  • Immunocytochemistry/ Immunofluorescence 10 ug/mL
  • Peptide ELISA Detection limit 1:128000
  • Western Blot 1:1000
• Application Notes: Western blot: Approx 75+80kDa band observed in lysates of cell line NIH-3T3 (calculated MW of 59.9 kDa according to NP_001013457.1). The observed molecular weight corresponds to earlier findings in literature (Khandjian et al Hum Mol Genet. 1998 Dec;7(13):2121-8.; PMID: 9817930). An additional band of unknown identity was also consistently observed at 37 kDa. This band was successfully blocked by incubation with the immunizing peptide.

Reactivity Notes

Expected from sequence similarity: Human, Rat, Canine

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for FXR1 Antibody

• fragile X mental retardation syndrome-related protein 1
• fragile X mental retardation, autosomal homolog 1
• FXR1P
• hFXR1p

Background

The protein encoded by this gene is an RNA binding protein that interacts with the functionally-similar proteins FMR1 and FXR2. These proteins shuttle between the nucleus and cytoplasm and associate with polyribosomes, predominantly with the 60S ribosomal subunit. Three transcript variants encoding different isoforms have been found for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: FXR1
• Entrez:
  • 8087(Human)
  • 14359(Mouse)
  • 361927(Rat)
• Uniprot:
  • NP_005078.2(Human)